Construction and identification of ATP4B-SV40T-IRES-GFP transgenic vector

AIM: To construct and identify ATP4B-SV40TIRES-GFP transgenic vector. METHODS: The mouse ATP4B gene promoter was amplified and inserted into IRES-GFP vector after the addition of restriction enzyme sites to result in ATP4B-IRES-GFP. After restriction enzyme digestion, the SV40T gene sequence was inserted into ATP4B-IRES-GFP. The resultant recombinant plasmid was identified by digestion with restriction enzymes PstI and Ase I. RESULTS: The recombinant plasmids ATP4BIRES-GFP and ATP4B-SV40T-IRES-GFP have been successfully constructed and can be used to generate transgenic mice. CONCLUSION: The recombinated plasmid ATP4B-SV40T-IRES-GFP was constructed successfully and it could be used to construct the transgenic mice with primary gastric cancer.