Compact RNA editors with small Cas13 proteins

Soumya Kannan; Han Altae-Tran; Xin Jin; Victoria J. Madigan; Rachel Oshiro; Kira S. Makarova; Eugene V. Koonin; Feng Zhang

doi:10.1038/s41587-021-01030-2

Compact RNA editors with small Cas13 proteins

Soumya Kannan
, Han Altae-Tran
, Xin Jin
, Victoria J. Madigan
, Rachel Oshiro
, Kira S. Makarova
, Eugene V. Koonin
, Feng Zhang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

128 Scopus citations

Abstract

CRISPR–Cas13 systems have been developed for precise RNA editing, and can potentially be used therapeutically when temporary changes are desirable or when DNA editing is challenging. We have identified and characterized an ultrasmall family of Cas13b proteins—Cas13bt—that can mediate mammalian transcript knockdown. We have engineered compact variants of REPAIR and RESCUE RNA editors by functionalizing Cas13bt with adenosine and cytosine deaminase domains, and demonstrated packaging of the editors within a single adeno-associated virus.

Original language	English
Pages (from-to)	194-197
Number of pages	4
Journal	Nature Biotechnology
Volume	40
Issue number	2
DOIs	https://doi.org/10.1038/s41587-021-01030-2
State	Published - Feb 2022
Externally published	Yes

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title = "Compact RNA editors with small Cas13 proteins",

abstract = "CRISPR–Cas13 systems have been developed for precise RNA editing, and can potentially be used therapeutically when temporary changes are desirable or when DNA editing is challenging. We have identified and characterized an ultrasmall family of Cas13b proteins—Cas13bt—that can mediate mammalian transcript knockdown. We have engineered compact variants of REPAIR and RESCUE RNA editors by functionalizing Cas13bt with adenosine and cytosine deaminase domains, and demonstrated packaging of the editors within a single adeno-associated virus.",

author = "Soumya Kannan and Han Altae-Tran and Xin Jin and Madigan, \{Victoria J.\} and Rachel Oshiro and Makarova, \{Kira S.\} and Koonin, \{Eugene V.\} and Feng Zhang",

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year = "2022",

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doi = "10.1038/s41587-021-01030-2",

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T1 - Compact RNA editors with small Cas13 proteins

AU - Kannan, Soumya

AU - Altae-Tran, Han

AU - Jin, Xin

AU - Madigan, Victoria J.

AU - Oshiro, Rachel

AU - Makarova, Kira S.

AU - Koonin, Eugene V.

AU - Zhang, Feng

PY - 2022/2

Y1 - 2022/2

N2 - CRISPR–Cas13 systems have been developed for precise RNA editing, and can potentially be used therapeutically when temporary changes are desirable or when DNA editing is challenging. We have identified and characterized an ultrasmall family of Cas13b proteins—Cas13bt—that can mediate mammalian transcript knockdown. We have engineered compact variants of REPAIR and RESCUE RNA editors by functionalizing Cas13bt with adenosine and cytosine deaminase domains, and demonstrated packaging of the editors within a single adeno-associated virus.

AB - CRISPR–Cas13 systems have been developed for precise RNA editing, and can potentially be used therapeutically when temporary changes are desirable or when DNA editing is challenging. We have identified and characterized an ultrasmall family of Cas13b proteins—Cas13bt—that can mediate mammalian transcript knockdown. We have engineered compact variants of REPAIR and RESCUE RNA editors by functionalizing Cas13bt with adenosine and cytosine deaminase domains, and demonstrated packaging of the editors within a single adeno-associated virus.

UR - https://www.scopus.com/pages/publications/85114001286

U2 - 10.1038/s41587-021-01030-2

DO - 10.1038/s41587-021-01030-2

M3 - 文章

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AN - SCOPUS:85114001286

SN - 1087-0156

VL - 40

SP - 194

EP - 197

JO - Nature Biotechnology

JF - Nature Biotechnology

IS - 2

ER -

Compact RNA editors with small Cas13 proteins

Abstract

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