Abstract
Objective: To obtain an effective immunization strategy by different types of HIV-2 gag recombinant vaccines against HIV-2. Methods: BALB/c mice were inoculated muscularly with HIV-2 gag recombinant DNA vaccine, or recombinant fowlpox virus, or both. Serum anti-HIV-2 antibody, spleen CD4+ and CD8+ T-cell counts and HIV-2-specific target-killing activity of spleen CTL in immunized mice were analyzed by ELISA, FACS and LDH release assay, respectively. Results: Serum HIV-2 specific antibody level and numbers of spleen CD4+ and CD8+ T-lymphocyte subgroups were detected higher by combined immunization than those of single immunizations, and HIV-2-specific target-killing activity of spleen CTL was stronger in combined immunization group. Conclusion: The strategy of priming with the recombinant DNA vaccine and boosting with the recombinant fowlpox virus shows a stronger specific cellular and humoral immune responses in mice.
| Original language | English |
|---|---|
| Pages (from-to) | 85-87 |
| Number of pages | 3 |
| Journal | Chinese Journal of Microbiology and Immunology |
| Volume | 26 |
| Issue number | 1 |
| State | Published - 30 Jan 2006 |
Keywords
- DNA vaccine
- Gag
- HIV-2
- Immunization strategy
- Recombinant fowlpox virus
