Coexpression of CdSe and CdSe/CdS quantum dots in live cells using molecular hyperspectral imaging technology

Qingli Li; Hui Peng; Jing Wang; Yiting Wang; Fangmin Guo

doi:10.1117/1.JBO.20.11.110504

Coexpression of CdSe and CdSe/CdS quantum dots in live cells using molecular hyperspectral imaging technology

Qingli Li^*
, Hui Peng
, Jing Wang
, Yiting Wang
, Fangmin Guo

^*Corresponding author for this work

East China Normal University

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A direct spatial and spectral observation of CdSe and CdSe/CdS quantum dots (QDs) as probes in live cells is performed by using a custom molecular hyperspectral imaging (MHI) system. Water-soluble CdSe and CdSe/CdS QDs are synthesized in aqueous solution under the assistance of high-intensity ultrasonic irradiation and incubated with colon cancer cells for bioimaging. Unlike the traditional fluorescence microscopy methods, MHI technology can identify QD probes according to their spectral signatures and generate coexpression and stain titer maps by a clustering method. The experimental results show that the MHI method has potential to unmix biomarkers by their spectral information, which opens up a pathway of optical multiplexing with many different QD probes.

Original language	English
Article number	110504
Journal	Journal of Biomedical Optics
Volume	20
Issue number	11
DOIs	https://doi.org/10.1117/1.JBO.20.11.110504
State	Published - 1 Nov 2015

Keywords

Spectral imaging
image detection systems
medical and biological imaging
quantum dots

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1117/1.JBO.20.11.110504

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title = "Coexpression of CdSe and CdSe/CdS quantum dots in live cells using molecular hyperspectral imaging technology",

abstract = "A direct spatial and spectral observation of CdSe and CdSe/CdS quantum dots (QDs) as probes in live cells is performed by using a custom molecular hyperspectral imaging (MHI) system. Water-soluble CdSe and CdSe/CdS QDs are synthesized in aqueous solution under the assistance of high-intensity ultrasonic irradiation and incubated with colon cancer cells for bioimaging. Unlike the traditional fluorescence microscopy methods, MHI technology can identify QD probes according to their spectral signatures and generate coexpression and stain titer maps by a clustering method. The experimental results show that the MHI method has potential to unmix biomarkers by their spectral information, which opens up a pathway of optical multiplexing with many different QD probes.",

keywords = "Spectral imaging, image detection systems, medical and biological imaging, quantum dots",

author = "Qingli Li and Hui Peng and Jing Wang and Yiting Wang and Fangmin Guo",

note = "Publisher Copyright: {\textcopyright} 2015 Society of Photo-Optical Instrumentation Engineers (SPIE).",

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doi = "10.1117/1.JBO.20.11.110504",

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T1 - Coexpression of CdSe and CdSe/CdS quantum dots in live cells using molecular hyperspectral imaging technology

AU - Li, Qingli

AU - Peng, Hui

AU - Wang, Jing

AU - Wang, Yiting

AU - Guo, Fangmin

PY - 2015/11/1

Y1 - 2015/11/1

N2 - A direct spatial and spectral observation of CdSe and CdSe/CdS quantum dots (QDs) as probes in live cells is performed by using a custom molecular hyperspectral imaging (MHI) system. Water-soluble CdSe and CdSe/CdS QDs are synthesized in aqueous solution under the assistance of high-intensity ultrasonic irradiation and incubated with colon cancer cells for bioimaging. Unlike the traditional fluorescence microscopy methods, MHI technology can identify QD probes according to their spectral signatures and generate coexpression and stain titer maps by a clustering method. The experimental results show that the MHI method has potential to unmix biomarkers by their spectral information, which opens up a pathway of optical multiplexing with many different QD probes.

AB - A direct spatial and spectral observation of CdSe and CdSe/CdS quantum dots (QDs) as probes in live cells is performed by using a custom molecular hyperspectral imaging (MHI) system. Water-soluble CdSe and CdSe/CdS QDs are synthesized in aqueous solution under the assistance of high-intensity ultrasonic irradiation and incubated with colon cancer cells for bioimaging. Unlike the traditional fluorescence microscopy methods, MHI technology can identify QD probes according to their spectral signatures and generate coexpression and stain titer maps by a clustering method. The experimental results show that the MHI method has potential to unmix biomarkers by their spectral information, which opens up a pathway of optical multiplexing with many different QD probes.

KW - Spectral imaging

KW - image detection systems

KW - medical and biological imaging

KW - quantum dots

UR - https://www.scopus.com/pages/publications/84948845619

U2 - 10.1117/1.JBO.20.11.110504

DO - 10.1117/1.JBO.20.11.110504

M3 - 文章

C2 - 26588112

AN - SCOPUS:84948845619

SN - 1083-3668

VL - 20

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

IS - 11

M1 - 110504

ER -

Coexpression of CdSe and CdSe/CdS quantum dots in live cells using molecular hyperspectral imaging technology

Abstract

Keywords

UN SDGs

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