Cloning of a cDNA encoding an Acanthamoeba castellanii PDI-like protein

Jie Min Wong; Erik Bateman

doi:10.1016/0378-1119(94)90880-X

Cloning of a cDNA encoding an Acanthamoeba castellanii PDI-like protein

Jie Min Wong, Erik Bateman^*

^*Corresponding author for this work

University of Vermont

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

We have cloned, from Acanthamoeba castellanii, the cDNA encoding a new member of the protein disulfide isomerase (PDI)-like protein family. The new PDI-like protein contains two highly conserved thioredeoxin-like domains, each about 100 amino acids in length. However, the A. castellanii PDI-like protein differs from other members in many aspects, including the overall organization and isoelectric point. Southern and Northern analyses demonstrate that the PDI-like protein is encoded by a single-copy gene which is transcribed to generate a 1500-nucleotide mRNA.

Original language	English
Pages (from-to)	175-179
Number of pages	5
Journal	Gene
Volume	150
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(94)90880-X
State	Published - 2 Dec 1994
Externally published	Yes

Keywords

Recombinant DNA
gene cloning
protein disulfide isomerase

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10.1016/0378-1119(94)90880-X

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title = "Cloning of a cDNA encoding an Acanthamoeba castellanii PDI-like protein",

abstract = "We have cloned, from Acanthamoeba castellanii, the cDNA encoding a new member of the protein disulfide isomerase (PDI)-like protein family. The new PDI-like protein contains two highly conserved thioredeoxin-like domains, each about 100 amino acids in length. However, the A. castellanii PDI-like protein differs from other members in many aspects, including the overall organization and isoelectric point. Southern and Northern analyses demonstrate that the PDI-like protein is encoded by a single-copy gene which is transcribed to generate a 1500-nucleotide mRNA.",

keywords = "Recombinant DNA, gene cloning, protein disulfide isomerase",

author = "Wong, \{Jie Min\} and Erik Bateman",

year = "1994",

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doi = "10.1016/0378-1119(94)90880-X",

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pages = "175--179",

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T1 - Cloning of a cDNA encoding an Acanthamoeba castellanii PDI-like protein

AU - Wong, Jie Min

AU - Bateman, Erik

PY - 1994/12/2

Y1 - 1994/12/2

N2 - We have cloned, from Acanthamoeba castellanii, the cDNA encoding a new member of the protein disulfide isomerase (PDI)-like protein family. The new PDI-like protein contains two highly conserved thioredeoxin-like domains, each about 100 amino acids in length. However, the A. castellanii PDI-like protein differs from other members in many aspects, including the overall organization and isoelectric point. Southern and Northern analyses demonstrate that the PDI-like protein is encoded by a single-copy gene which is transcribed to generate a 1500-nucleotide mRNA.

AB - We have cloned, from Acanthamoeba castellanii, the cDNA encoding a new member of the protein disulfide isomerase (PDI)-like protein family. The new PDI-like protein contains two highly conserved thioredeoxin-like domains, each about 100 amino acids in length. However, the A. castellanii PDI-like protein differs from other members in many aspects, including the overall organization and isoelectric point. Southern and Northern analyses demonstrate that the PDI-like protein is encoded by a single-copy gene which is transcribed to generate a 1500-nucleotide mRNA.

KW - Recombinant DNA

KW - gene cloning

KW - protein disulfide isomerase

UR - https://www.scopus.com/pages/publications/0027987374

U2 - 10.1016/0378-1119(94)90880-X

DO - 10.1016/0378-1119(94)90880-X

M3 - 文章

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AN - SCOPUS:0027987374

SN - 0378-1119

VL - 150

SP - 175

EP - 179

JO - Gene

JF - Gene

IS - 1

ER -

Cloning of a cDNA encoding an Acanthamoeba castellanii PDI-like protein

Abstract

Keywords

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