Cloning, expression and cellular localization of the Doublesex gene in the water flea, Daphnia carinata, during different developmental stages

Mingqing Zhang; Haixia Li; Ajing Liu; Donglei Wu; Danli Wang; Yunlong Zhao

doi:10.1016/j.gene.2014.08.026

Cloning, expression and cellular localization of the Doublesex gene in the water flea, Daphnia carinata, during different developmental stages

Mingqing Zhang
, Haixia Li
, Ajing Liu
, Donglei Wu
, Danli Wang
, Yunlong Zhao^*

^*Corresponding author for this work

School of Life Sciences

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

In this study, one of Doublesex genes from the common freshwater cladoceran Daphnia carinata, designated DapcaDsx1, was cloned using primers based on homologous sequences and rapid amplification of cDNA ends (RACE). qPCR was employed to quantify differences in DapcaDsx1 expression between the different sexual phases, with expression levels being higher in sexual females. The role of DapcaDsx1 in the reproductive transformation was further investigated in parthenogenetic-phase females and sexual-phase females using whole-mount in situ hybridization. This cellular localization study showed specific expression of DapcaDsx1 in the thoracic segments, second antenna and part of the ventral carapace. Higher expression levels were exhibited in sexual females compared to parthenogenetic females. This suggests that the DapcaDsx1 gene plays significant roles in switching modes of reproduction and during sexual differentiation.

Original language	English
Pages (from-to)	185-192
Number of pages	8
Journal	Gene
Volume	550
Issue number	2
DOIs	https://doi.org/10.1016/j.gene.2014.08.026
State	Published - 2014

Keywords

Daphnia carinata
Doublesex gene
Expression
Reproductive transformation
Whole-mount in situ hybridization

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10.1016/j.gene.2014.08.026

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@article{1029884f9206458e95947713ad99225f,

title = "Cloning, expression and cellular localization of the Doublesex gene in the water flea, Daphnia carinata, during different developmental stages",

abstract = "In this study, one of Doublesex genes from the common freshwater cladoceran Daphnia carinata, designated DapcaDsx1, was cloned using primers based on homologous sequences and rapid amplification of cDNA ends (RACE). qPCR was employed to quantify differences in DapcaDsx1 expression between the different sexual phases, with expression levels being higher in sexual females. The role of DapcaDsx1 in the reproductive transformation was further investigated in parthenogenetic-phase females and sexual-phase females using whole-mount in situ hybridization. This cellular localization study showed specific expression of DapcaDsx1 in the thoracic segments, second antenna and part of the ventral carapace. Higher expression levels were exhibited in sexual females compared to parthenogenetic females. This suggests that the DapcaDsx1 gene plays significant roles in switching modes of reproduction and during sexual differentiation.",

keywords = "Daphnia carinata, Doublesex gene, Expression, Reproductive transformation, Whole-mount in situ hybridization",

author = "Mingqing Zhang and Haixia Li and Ajing Liu and Donglei Wu and Danli Wang and Yunlong Zhao",

note = "Publisher Copyright: {\textcopyright} 2014 Elsevier B.V.",

year = "2014",

doi = "10.1016/j.gene.2014.08.026",

language = "英语",

volume = "550",

pages = "185--192",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Cloning, expression and cellular localization of the Doublesex gene in the water flea, Daphnia carinata, during different developmental stages

AU - Zhang, Mingqing

AU - Li, Haixia

AU - Liu, Ajing

AU - Wu, Donglei

AU - Wang, Danli

AU - Zhao, Yunlong

PY - 2014

Y1 - 2014

N2 - In this study, one of Doublesex genes from the common freshwater cladoceran Daphnia carinata, designated DapcaDsx1, was cloned using primers based on homologous sequences and rapid amplification of cDNA ends (RACE). qPCR was employed to quantify differences in DapcaDsx1 expression between the different sexual phases, with expression levels being higher in sexual females. The role of DapcaDsx1 in the reproductive transformation was further investigated in parthenogenetic-phase females and sexual-phase females using whole-mount in situ hybridization. This cellular localization study showed specific expression of DapcaDsx1 in the thoracic segments, second antenna and part of the ventral carapace. Higher expression levels were exhibited in sexual females compared to parthenogenetic females. This suggests that the DapcaDsx1 gene plays significant roles in switching modes of reproduction and during sexual differentiation.

AB - In this study, one of Doublesex genes from the common freshwater cladoceran Daphnia carinata, designated DapcaDsx1, was cloned using primers based on homologous sequences and rapid amplification of cDNA ends (RACE). qPCR was employed to quantify differences in DapcaDsx1 expression between the different sexual phases, with expression levels being higher in sexual females. The role of DapcaDsx1 in the reproductive transformation was further investigated in parthenogenetic-phase females and sexual-phase females using whole-mount in situ hybridization. This cellular localization study showed specific expression of DapcaDsx1 in the thoracic segments, second antenna and part of the ventral carapace. Higher expression levels were exhibited in sexual females compared to parthenogenetic females. This suggests that the DapcaDsx1 gene plays significant roles in switching modes of reproduction and during sexual differentiation.

KW - Daphnia carinata

KW - Doublesex gene

KW - Expression

KW - Reproductive transformation

KW - Whole-mount in situ hybridization

UR - https://www.scopus.com/pages/publications/84921681898

U2 - 10.1016/j.gene.2014.08.026

DO - 10.1016/j.gene.2014.08.026

M3 - 文章

C2 - 25130908

AN - SCOPUS:84921681898

SN - 0378-1119

VL - 550

SP - 185

EP - 192

JO - Gene

JF - Gene

IS - 2

ER -

Cloning, expression and cellular localization of the Doublesex gene in the water flea, Daphnia carinata, during different developmental stages

Abstract

Keywords

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