TY - JOUR
T1 - Cloning and expression profiling of a cuticular protein gene in Daphnia carinata
AU - Liu, Ajing
AU - Zhang, Mingqing
AU - Kong, Ling
AU - Wu, Donglei
AU - Weng, Xianlong
AU - Wang, Danli
AU - Zhao, Yunlong
PY - 2014/6
Y1 - 2014/6
N2 - The cladoceran Daphnia carinata undergoes an unusual transition from asexual to sexual reproduction in response to environmental stimuli. Previously, a D. carinata cuticular protein (CP) was identified in an EST library. In this study, the full-length CP cDNA was cloned and sequenced (GenBank accession number: KF551931), and the expression levels in different reproductive states were assessed. Parthenogenetic and sexual female D. carinata were isolated, and CP expression was investigated using semiquantitative reverse transcription polymerase chain reaction (RT-PCR). CP was expressed during both reproductive stages, but expression was higher in sexual females. Cellular localization was also investigated using digoxin-labeled RNA probes in RNA whole-mount in situ hybridization assays, and CP was mainly expressed in the first pair of thoracic appendages, the surface of the head, shell spines, and other parts of the epidermis in parthenogenetic organisms. In contrast, CP expression was restricted to the thoracic appendages in sexual females.
AB - The cladoceran Daphnia carinata undergoes an unusual transition from asexual to sexual reproduction in response to environmental stimuli. Previously, a D. carinata cuticular protein (CP) was identified in an EST library. In this study, the full-length CP cDNA was cloned and sequenced (GenBank accession number: KF551931), and the expression levels in different reproductive states were assessed. Parthenogenetic and sexual female D. carinata were isolated, and CP expression was investigated using semiquantitative reverse transcription polymerase chain reaction (RT-PCR). CP was expressed during both reproductive stages, but expression was higher in sexual females. Cellular localization was also investigated using digoxin-labeled RNA probes in RNA whole-mount in situ hybridization assays, and CP was mainly expressed in the first pair of thoracic appendages, the surface of the head, shell spines, and other parts of the epidermis in parthenogenetic organisms. In contrast, CP expression was restricted to the thoracic appendages in sexual females.
KW - Cuticular protein
KW - Daphnia carinata
KW - Expression analysis
KW - Gene cloning
KW - Whole-mount in situ hybridization
UR - https://www.scopus.com/pages/publications/84902830532
U2 - 10.1007/s00427-014-0469-9
DO - 10.1007/s00427-014-0469-9
M3 - 文章
C2 - 24619580
AN - SCOPUS:84902830532
SN - 0949-944X
VL - 224
SP - 129
EP - 135
JO - Development Genes and Evolution
JF - Development Genes and Evolution
IS - 3
ER -