TY - JOUR
T1 - Cloning and characterisation of Na+/K+-ATPase and carbonic anhydrase from oriental river prawn Macrobrachium nipponense
AU - Huang, Youhui
AU - Liu, Zhiquan
AU - Li, Yiming
AU - Wu, Donglei
AU - Zhang, Meng
AU - Zhao, Yunlong
N1 - Publisher Copyright:
© 2019
PY - 2019/5/15
Y1 - 2019/5/15
N2 - Na+/K+-ATPase (NKA) and carbonic anhydrase (CA) are ion transporters that play important roles in osmotic pressure balance in crustaceans. Oriental river prawn (Macrobrachium nipponense) is one of the most economically important shrimps in China. This species can live in freshwater and low-salt water, but the molecular mechanism of salinity regulation is unclear. In this study, full-length cDNAs of M. nipponense α-NKA and CA were obtained by rapid amplification of cDNA ends (RACE)-PCR (GenBank accession numbers MH378774 and MH827971, respectively), and characterised. Full-length cDNAs of Mnα-NKA (3778 bp) and MnCA (1407 bp) contain open reading frames of 3030 bp and 930 bp, respectively, encoding polypeptides of 1009 and 930 amino acids. Bioinformatic analysis showed that Mnα-NKA and MnCA harbour the representative features of NKAs and CAs, and share high homology with orthologs in other crustaceans. Quantitative real-time PCR revealed that Mnα-NKA and MnCA were expressed in various tissues, with highest expression in posterior gills and hepatopancreas. Both Mnα-NKA and MnCA were up-regulated in response to salinity acclimation, but expression patterns differed. Our results provide a theoretical basis for the mechanism regulating salinity acclimation in M. nipponense.
AB - Na+/K+-ATPase (NKA) and carbonic anhydrase (CA) are ion transporters that play important roles in osmotic pressure balance in crustaceans. Oriental river prawn (Macrobrachium nipponense) is one of the most economically important shrimps in China. This species can live in freshwater and low-salt water, but the molecular mechanism of salinity regulation is unclear. In this study, full-length cDNAs of M. nipponense α-NKA and CA were obtained by rapid amplification of cDNA ends (RACE)-PCR (GenBank accession numbers MH378774 and MH827971, respectively), and characterised. Full-length cDNAs of Mnα-NKA (3778 bp) and MnCA (1407 bp) contain open reading frames of 3030 bp and 930 bp, respectively, encoding polypeptides of 1009 and 930 amino acids. Bioinformatic analysis showed that Mnα-NKA and MnCA harbour the representative features of NKAs and CAs, and share high homology with orthologs in other crustaceans. Quantitative real-time PCR revealed that Mnα-NKA and MnCA were expressed in various tissues, with highest expression in posterior gills and hepatopancreas. Both Mnα-NKA and MnCA were up-regulated in response to salinity acclimation, but expression patterns differed. Our results provide a theoretical basis for the mechanism regulating salinity acclimation in M. nipponense.
KW - Macrobrachium nipponense
KW - MnCA
KW - Mnα-NKA
UR - https://www.scopus.com/pages/publications/85061637087
U2 - 10.1016/j.ijbiomac.2019.02.098
DO - 10.1016/j.ijbiomac.2019.02.098
M3 - 文章
C2 - 30784852
AN - SCOPUS:85061637087
SN - 0141-8130
VL - 129
SP - 809
EP - 817
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -