Characterization of Cdc2 kinase in the red claw crayfish (Cherax quadricarinatus): Evidence for its role in regulating oogenesis

Lan mei Wang; Di Zuo; Wei wei Lv; Dan li Wang; A. jing Liu; Yunlong Zhao

doi:10.1016/j.gene.2012.11.082

Characterization of Cdc2 kinase in the red claw crayfish (Cherax quadricarinatus): Evidence for its role in regulating oogenesis

Lan mei Wang, Di Zuo, Wei wei Lv, Dan li Wang, A. jing Liu, Yunlong Zhao

School of Life Sciences

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Cdc2 kinase is a catalytic subunit of the maturation-promoting factor (MPF), a central factor for inducing the meiotic maturation of oocytes. MPF has been studied in a wide variety of animal species; however, its expression in crustaceans is poorly characterized. In this study, a complete cDNA sequence of Cdc2 kinase was cloned from the red claw crayfish, Cherax quadricarinatus, and its spatiotemporal expression profiles were analyzed. The Cdc2 cDNA (1769. bp) encodes for a 299 amino acid protein with a calculated molecular weight of 34.7. kDa. Quantitative real-time PCR demonstrated that Cdc2 mRNA was expressed mainly in the ovary tissue and the expression decreased as the ovaries developed. Immunohistochemistry analysis revealed that the Cdc2 protein relocated from the cytoplasm to the nucleus during oogenesis. These findings suggest that Cdc2 kinase may play an important role in the gametogenesis and gonad development in C. quadricarinatus.

Original language	English
Pages (from-to)	258-265
Number of pages	8
Journal	Gene
Volume	515
Issue number	2
DOIs	https://doi.org/10.1016/j.gene.2012.11.082
State	Published - 25 Feb 2013

Keywords

CDNA cloning
Cdc2 kinase
Cherax quadricarinatus
Expression
Oogenesis

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@article{e312ebff7a634914a9dee94180e6ec6c,

title = "Characterization of Cdc2 kinase in the red claw crayfish (Cherax quadricarinatus): Evidence for its role in regulating oogenesis",

abstract = "Cdc2 kinase is a catalytic subunit of the maturation-promoting factor (MPF), a central factor for inducing the meiotic maturation of oocytes. MPF has been studied in a wide variety of animal species; however, its expression in crustaceans is poorly characterized. In this study, a complete cDNA sequence of Cdc2 kinase was cloned from the red claw crayfish, Cherax quadricarinatus, and its spatiotemporal expression profiles were analyzed. The Cdc2 cDNA (1769. bp) encodes for a 299 amino acid protein with a calculated molecular weight of 34.7. kDa. Quantitative real-time PCR demonstrated that Cdc2 mRNA was expressed mainly in the ovary tissue and the expression decreased as the ovaries developed. Immunohistochemistry analysis revealed that the Cdc2 protein relocated from the cytoplasm to the nucleus during oogenesis. These findings suggest that Cdc2 kinase may play an important role in the gametogenesis and gonad development in C. quadricarinatus.",

keywords = "CDNA cloning, Cdc2 kinase, Cherax quadricarinatus, Expression, Oogenesis",

author = "Wang, \{Lan mei\} and Di Zuo and Lv, \{Wei wei\} and Wang, \{Dan li\} and Liu, \{A. jing\} and Yunlong Zhao",

year = "2013",

month = feb,

day = "25",

doi = "10.1016/j.gene.2012.11.082",

language = "英语",

volume = "515",

pages = "258--265",

journal = "Gene",

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publisher = "Elsevier B.V.",

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T1 - Characterization of Cdc2 kinase in the red claw crayfish (Cherax quadricarinatus)

T2 - Evidence for its role in regulating oogenesis

AU - Wang, Lan mei

AU - Zuo, Di

AU - Lv, Wei wei

AU - Wang, Dan li

AU - Liu, A. jing

AU - Zhao, Yunlong

PY - 2013/2/25

Y1 - 2013/2/25

N2 - Cdc2 kinase is a catalytic subunit of the maturation-promoting factor (MPF), a central factor for inducing the meiotic maturation of oocytes. MPF has been studied in a wide variety of animal species; however, its expression in crustaceans is poorly characterized. In this study, a complete cDNA sequence of Cdc2 kinase was cloned from the red claw crayfish, Cherax quadricarinatus, and its spatiotemporal expression profiles were analyzed. The Cdc2 cDNA (1769. bp) encodes for a 299 amino acid protein with a calculated molecular weight of 34.7. kDa. Quantitative real-time PCR demonstrated that Cdc2 mRNA was expressed mainly in the ovary tissue and the expression decreased as the ovaries developed. Immunohistochemistry analysis revealed that the Cdc2 protein relocated from the cytoplasm to the nucleus during oogenesis. These findings suggest that Cdc2 kinase may play an important role in the gametogenesis and gonad development in C. quadricarinatus.

AB - Cdc2 kinase is a catalytic subunit of the maturation-promoting factor (MPF), a central factor for inducing the meiotic maturation of oocytes. MPF has been studied in a wide variety of animal species; however, its expression in crustaceans is poorly characterized. In this study, a complete cDNA sequence of Cdc2 kinase was cloned from the red claw crayfish, Cherax quadricarinatus, and its spatiotemporal expression profiles were analyzed. The Cdc2 cDNA (1769. bp) encodes for a 299 amino acid protein with a calculated molecular weight of 34.7. kDa. Quantitative real-time PCR demonstrated that Cdc2 mRNA was expressed mainly in the ovary tissue and the expression decreased as the ovaries developed. Immunohistochemistry analysis revealed that the Cdc2 protein relocated from the cytoplasm to the nucleus during oogenesis. These findings suggest that Cdc2 kinase may play an important role in the gametogenesis and gonad development in C. quadricarinatus.

KW - CDNA cloning

KW - Cdc2 kinase

KW - Cherax quadricarinatus

KW - Expression

KW - Oogenesis

UR - https://www.scopus.com/pages/publications/84872499788

U2 - 10.1016/j.gene.2012.11.082

DO - 10.1016/j.gene.2012.11.082

M3 - 文章

C2 - 23266620

AN - SCOPUS:84872499788

SN - 0378-1119

VL - 515

SP - 258

EP - 265

JO - Gene

JF - Gene

IS - 2

ER -

Characterization of Cdc2 kinase in the red claw crayfish (Cherax quadricarinatus): Evidence for its role in regulating oogenesis

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Keywords

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