Characterization of an Anti-Thioredoxin Monoclonal Antibody

Wei Zhang; Wang Zhang; Yanchun Tang; Jing Zhang; Jian Ning Liu

doi:10.1007/s10529-005-5333-6

Characterization of an Anti-Thioredoxin Monoclonal Antibody

Wei Zhang
, Wang Zhang
, Yanchun Tang
, Jing Zhang
, Jian Ning Liu^*

^*Corresponding author for this work

Nanjing University

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

An anti-E. coli thioredoxin monoclonal antibody, IMM-3C6, which showed high specificity to thioredoxin as assessed by indirect ELISA, was generated using hybridoma technology. The affinity constant of IMM-3C6 to thioredoxin was 0.40×10⁹ m ^-1 and its sensitivity to thioredoxin fusion protein in dot blotting was 50 ng. In sandwich ELISA, it detected thioredoxin fusion protein between 16 and 150 ng/ml. By using IMM-3C6 as the ligand, thioredoxin fusion protein was successfully purified by affinity chromatography. IMM-3C6 was confirmed to be a useful tool for immunoassay and purification of thioredoxin fusion proteins.

Original language	English
Pages (from-to)	183-188
Number of pages	6
Journal	Biotechnology Letters
Volume	28
Issue number	3
DOIs	https://doi.org/10.1007/s10529-005-5333-6
State	Published - Feb 2006
Externally published	Yes

Keywords

Affinity chromatography
Monoclonal antibody
Thioredoxin
Western blotting

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10.1007/s10529-005-5333-6

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title = "Characterization of an Anti-Thioredoxin Monoclonal Antibody",

abstract = "An anti-E. coli thioredoxin monoclonal antibody, IMM-3C6, which showed high specificity to thioredoxin as assessed by indirect ELISA, was generated using hybridoma technology. The affinity constant of IMM-3C6 to thioredoxin was 0.40×109 m -1 and its sensitivity to thioredoxin fusion protein in dot blotting was 50 ng. In sandwich ELISA, it detected thioredoxin fusion protein between 16 and 150 ng/ml. By using IMM-3C6 as the ligand, thioredoxin fusion protein was successfully purified by affinity chromatography. IMM-3C6 was confirmed to be a useful tool for immunoassay and purification of thioredoxin fusion proteins.",

keywords = "Affinity chromatography, Monoclonal antibody, Thioredoxin, Western blotting",

author = "Wei Zhang and Wang Zhang and Yanchun Tang and Jing Zhang and Liu, \{Jian Ning\}",

year = "2006",

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doi = "10.1007/s10529-005-5333-6",

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T1 - Characterization of an Anti-Thioredoxin Monoclonal Antibody

AU - Zhang, Wei

AU - Zhang, Wang

AU - Tang, Yanchun

AU - Zhang, Jing

AU - Liu, Jian Ning

PY - 2006/2

Y1 - 2006/2

N2 - An anti-E. coli thioredoxin monoclonal antibody, IMM-3C6, which showed high specificity to thioredoxin as assessed by indirect ELISA, was generated using hybridoma technology. The affinity constant of IMM-3C6 to thioredoxin was 0.40×109 m -1 and its sensitivity to thioredoxin fusion protein in dot blotting was 50 ng. In sandwich ELISA, it detected thioredoxin fusion protein between 16 and 150 ng/ml. By using IMM-3C6 as the ligand, thioredoxin fusion protein was successfully purified by affinity chromatography. IMM-3C6 was confirmed to be a useful tool for immunoassay and purification of thioredoxin fusion proteins.

AB - An anti-E. coli thioredoxin monoclonal antibody, IMM-3C6, which showed high specificity to thioredoxin as assessed by indirect ELISA, was generated using hybridoma technology. The affinity constant of IMM-3C6 to thioredoxin was 0.40×109 m -1 and its sensitivity to thioredoxin fusion protein in dot blotting was 50 ng. In sandwich ELISA, it detected thioredoxin fusion protein between 16 and 150 ng/ml. By using IMM-3C6 as the ligand, thioredoxin fusion protein was successfully purified by affinity chromatography. IMM-3C6 was confirmed to be a useful tool for immunoassay and purification of thioredoxin fusion proteins.

KW - Affinity chromatography

KW - Monoclonal antibody

KW - Thioredoxin

KW - Western blotting

UR - https://www.scopus.com/pages/publications/32944468282

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DO - 10.1007/s10529-005-5333-6

M3 - 文章

C2 - 16489496

AN - SCOPUS:32944468282

SN - 0141-5492

VL - 28

SP - 183

EP - 188

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 3

ER -

Characterization of an Anti-Thioredoxin Monoclonal Antibody

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Keywords

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