TY - JOUR
T1 - Characterization and optimization of production of bacterial cellulose from strain CGMCC 17276 based on whole-genome analysis
AU - Lu, Tingfen
AU - Gao, Hongliang
AU - Liao, Bowen
AU - Wu, Jiajing
AU - Zhang, Wei
AU - Huang, Jie
AU - Liu, Mingyao
AU - Huang, Jing
AU - Chang, Zhongyi
AU - Jin, Mingfei
AU - Yi, Zhengfang
AU - Jiang, Deming
N1 - Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2020/3/15
Y1 - 2020/3/15
N2 - Bacterial cellulose (BC) has received considerable attention as an environment-friendly, biodegradable nanomaterial. In this study, the strain Komagataeibacter sp. nov. CGMCC 17276, which showed rapid cell growth and high BC-production ability, was isolated and classified into a novel species in the Komagataeibacter genus. Four BC synthase operons were annotated using whole-genome analysis, partially explaining the high BC yield of strain CGMCC 17276. Operons bcs Ⅱ and bcs Ⅲ showed high transcriptional levels under static and agitated culture conditions, indicating their importance in BC synthesis. Of the eight suitable carbon sources identified by whole-genome analysis, the highest BC production was achieved using glycerol as a single carbon source. Finally, waste glycerol was successfully used as an eco-friendly and sustainable strategy for BC production. This study provides valuable insights into the mechanism of BC synthesis, genetic structure of BC-producing strains, and industrialization of BC production using an eco-friendly and low-cost strategy.
AB - Bacterial cellulose (BC) has received considerable attention as an environment-friendly, biodegradable nanomaterial. In this study, the strain Komagataeibacter sp. nov. CGMCC 17276, which showed rapid cell growth and high BC-production ability, was isolated and classified into a novel species in the Komagataeibacter genus. Four BC synthase operons were annotated using whole-genome analysis, partially explaining the high BC yield of strain CGMCC 17276. Operons bcs Ⅱ and bcs Ⅲ showed high transcriptional levels under static and agitated culture conditions, indicating their importance in BC synthesis. Of the eight suitable carbon sources identified by whole-genome analysis, the highest BC production was achieved using glycerol as a single carbon source. Finally, waste glycerol was successfully used as an eco-friendly and sustainable strategy for BC production. This study provides valuable insights into the mechanism of BC synthesis, genetic structure of BC-producing strains, and industrialization of BC production using an eco-friendly and low-cost strategy.
KW - Bacterial cellulose
KW - Komagataeibacter
KW - Optimization of fermentation medium
KW - Waste glycerol
KW - Whole-genome analysis
UR - https://www.scopus.com/pages/publications/85077382313
U2 - 10.1016/j.carbpol.2019.115788
DO - 10.1016/j.carbpol.2019.115788
M3 - 文章
C2 - 31952596
AN - SCOPUS:85077382313
SN - 0144-8617
VL - 232
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
M1 - 115788
ER -