Abstract
A fowlpox virus (FPV) transferring vector pUTA2-gag-gp105 was constructed by inserting HIV-2 gag-gp105 chimeric gene to the downstream of a synthetic complex promoter ATI-p7.5 × 20 of vector pUTA2. Transfection was then carried out, and recombinant FPV (rFPV) was screened by 5'-bromo-deoxyuridine (BrdU), genome PCR and Western blot detection. Hereditary stability of the rFPV was detected. BALB/c mice were immunized with rFPV by muscular injection. Anti-HIV-2 antibody, CD4+ and CD8+ T-cell count and specific target-killing activity of spleen CTL in immunized mice were analyzed by ELISA, FACS and LDH release assay, respectively. The results showed that a recombinant virus rFPV-gg which could stably express HIV-2 chimeric protein gag-gp105 was obtained. The HIV-2 specific antibody was detected from the immunized BALB/c mice. The numbers of CD4+ and CD8+ subgroup of spleen T lymphocyte were higher in immunization group than those in controls. HIV-2-Specific target-killing activity of spleen CTL was observed in immunized mice. It was concluded that the rFPV-gg may elicit specific cellular and humoral immune reactions in mice.
| Original language | English |
|---|---|
| Pages (from-to) | 730-734 |
| Number of pages | 5 |
| Journal | Gaojishu Tongxin/Chinese High Technology Letters |
| Volume | 16 |
| Issue number | 7 |
| State | Published - Jul 2006 |
Keywords
- Fowlpox virus
- HIV-2
- Immune response
- Recombinant
- Structural gene
