Brain metabolite profiles provide insight into mechanisms for behavior sexual dimorphisms in zebrafish (Danio rerio)

  • Jing Xiang
  • , Rui ying Guo
  • , Ting Wang
  • , Nan Zhang
  • , Xian rui Chen
  • , Er chao Li
  • , Ji liang Zhang*
  • *Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The zebrafish (Danio rerio) has historically been a useful model for research in genetics, ecology, biology, toxicology, and neurobehavior. Zebrafish have been demonstrated to have brain sexual dimorphism. However, the sexual dimorphism of zebrafish behavior demands our attention, particularly. To evaluate the behavior and brain sexual dimorphisms in zebrafish, this study assessed sex differences in adult D. rerio in four behavioral domains, including aggression, fear, anxiety, and shoaling, and further compared with metabolites in the brain tissue of females and males. Our findings showed that aggression, fear, anxiety and shoaling behaviors were significantly sexually dimorphic. Interestingly, we also show through a novel data analysis method, that the female zebrafish exhibited significantly increased shoaling behavior when shoaled with male zebrafish groups and, for the first time, we offer evidence that male shoals are beneficial in dramatically alleviating anxiety in zebrafish. In addition, there were significant changes in metabolites in zebrafish brain tissue between the sexes. Furthermore, zebrafish behavioral sexual dimorphism may be associated with brain sexual dimorphism, with significant differences in brain metabolites. Therefore, to prevent the influence or even bias of behavioral sex differences on results, it is suggested that behavioral studies or behavioral-based other relevant investigations consider sexual dimorphism of behavior and brain.

Original languageEnglish
Article number114132
JournalPhysiology and Behavior
Volume263
DOIs
StatePublished - 1 May 2023
Externally publishedYes

Keywords

  • Aggression
  • Anxiety
  • Brain metabolites
  • Fear
  • Sex differences
  • Shoaling

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