Boron-substituted rhodamine for ratiometric monitoring dynamic of H₂O₂ and HOCl in vivo

Simultaneously analyzing different species of reactive oxygen species (ROS) with substantial selectivity and sensitivity is eagerly demanded exploring the related physiological and pathological processes. A compact and robust boron-substituted rhodamine scaffold, MMBR with two integrated reaction sites, was developed for simultaneously monitoring H₂O₂ and HOCl in a ratiometric manner. Both analytes were sensitively monitored with a low limit of detection (in nM level) and a wide linear range. The endogenous and exogenous HOCl and H₂O₂ were evaluated by the developed probe in living cells and zebrafish. Furthermore, ROS dynamics in both elesclomol-treated cancer cells and wounded zebrafish were vividly manifested by MMBR.