TY - JOUR
T1 - Biodegradation of phenanthrene by Rhizobium petrolearium SL-1
AU - Huang, X.
AU - Shi, J.
AU - Cui, C.
AU - Yin, H.
AU - Zhang, R.
AU - Ma, X.
AU - Zhang, X.
N1 - Publisher Copyright:
© 2016 The Society for Applied Microbiology
PY - 2016/12/1
Y1 - 2016/12/1
N2 - Aims: We aimed to investigate the phenanthrene degradation characteristics and possible phenanthrene degradation pathways of Rhizobium petrolearium SL-1. Methods and Results: Using high-performance liquid chromatography (HPLC), UV-visible and gas chromatography-mass spectrometry (GC-MS) analysis, the phenanthrene-degrading properties and metabolites of Rh. petrolearium SL-1 were analysed, and then combined with genome-based analysis to elucidate the possible biodegradation pathway of phenanthrene in Rh. petrolearium SL-1. The results of the analyses showed that phenanthrene (100 mg l−1) was completely degraded by strain SL-1 at 35°C, 0·02% salinity and pH 9·0 within 3 days. A wide range of polycyclic aromatic hydrocarbons, including naphthalene, fluorene, anthracene and pyrene, could also be degraded by this strain. Conclusions: Based on the identified metabolites, the utilization of probable intermediates and the presence of putative phenanthrene catabolic genes, we concluded that phenanthrene was degraded via two different routes, namely the ‘naphthalene’ and the ‘phthalic acid’ routes. Significance and Impact of the Study: For the first time, this study shows the degradation pathway of phenanthrene by a Rhizobium strain. Because of its excellent stress resistance, metabolic versatility, high degradation efficiency and potential application in phytoremediation, Rh. petrolearium SL-1 is a potential candidate for the bioremediation of polycyclic aromatic hydrocarbons-contaminated areas.
AB - Aims: We aimed to investigate the phenanthrene degradation characteristics and possible phenanthrene degradation pathways of Rhizobium petrolearium SL-1. Methods and Results: Using high-performance liquid chromatography (HPLC), UV-visible and gas chromatography-mass spectrometry (GC-MS) analysis, the phenanthrene-degrading properties and metabolites of Rh. petrolearium SL-1 were analysed, and then combined with genome-based analysis to elucidate the possible biodegradation pathway of phenanthrene in Rh. petrolearium SL-1. The results of the analyses showed that phenanthrene (100 mg l−1) was completely degraded by strain SL-1 at 35°C, 0·02% salinity and pH 9·0 within 3 days. A wide range of polycyclic aromatic hydrocarbons, including naphthalene, fluorene, anthracene and pyrene, could also be degraded by this strain. Conclusions: Based on the identified metabolites, the utilization of probable intermediates and the presence of putative phenanthrene catabolic genes, we concluded that phenanthrene was degraded via two different routes, namely the ‘naphthalene’ and the ‘phthalic acid’ routes. Significance and Impact of the Study: For the first time, this study shows the degradation pathway of phenanthrene by a Rhizobium strain. Because of its excellent stress resistance, metabolic versatility, high degradation efficiency and potential application in phytoremediation, Rh. petrolearium SL-1 is a potential candidate for the bioremediation of polycyclic aromatic hydrocarbons-contaminated areas.
KW - Rhizobium
KW - biodegradation
KW - degrading properties
KW - metabolic pathway
KW - phenanthrene
UR - https://www.scopus.com/pages/publications/84995685185
U2 - 10.1111/jam.13292
DO - 10.1111/jam.13292
M3 - 文章
C2 - 27614183
AN - SCOPUS:84995685185
SN - 1364-5072
VL - 121
SP - 1616
EP - 1626
JO - Journal of Applied Microbiology
JF - Journal of Applied Microbiology
IS - 6
ER -