Atypical OmpR/PhoB subfamily response regulator glnr of actinomycetes functions as a homodimer, stabilized by the unphosphorylated conserved Asp-focused charge interactions

Wei Lin; Ying Wang; Xiaobiao Han; Zilong Zhang; Chengyuan Wang; Jin Wang; Huaiyu Yang; Yinhua Lu; Weihong Jiang; Guo Ping Zhao; Peng Zhang

doi:10.1074/jbc.M113.543504

Atypical OmpR/PhoB subfamily response regulator glnr of actinomycetes functions as a homodimer, stabilized by the unphosphorylated conserved Asp-focused charge interactions

Wei Lin
, Ying Wang
, Xiaobiao Han
, Zilong Zhang
, Chengyuan Wang
, Jin Wang
, Huaiyu Yang
, Yinhua Lu
, Weihong Jiang
, Guo Ping Zhao
, Peng Zhang^*

^*Corresponding author for this work

Chinese Academy of Sciences
Fudan University
CAS - Shanghai Institute of Materia Medica
Chinese National Human Genome Center
Prince of Wales Hospital Hong Kong

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Background: Orphan response transcription factor GlnR regulates nitrogen metabolism in important actinomycetes. Results: GlnR has no typical "phosphorylation pocket," where the only conserved Asp is unphosphorylated but is essential for functional homodimerization. Conclusion: Actinomycete GlnR is an atypical response regulator functioning as a homodimer. Significance: Conserved Asp-focused charge interactions of actinomycete GlnR are probably the mechanism that stabilizes the homodimer for physiological function.

Original language	English
Pages (from-to)	15413-15425
Number of pages	13
Journal	Journal of Biological Chemistry
Volume	289
Issue number	22
DOIs	https://doi.org/10.1074/jbc.M113.543504
State	Published - 30 May 2014
Externally published	Yes

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Lin, W., Wang, Y., Han, X., Zhang, Z., Wang, C., Wang, J., Yang, H., Lu, Y., Jiang, W., Zhao, G. P., & Zhang, P. (2014). Atypical OmpR/PhoB subfamily response regulator glnr of actinomycetes functions as a homodimer, stabilized by the unphosphorylated conserved Asp-focused charge interactions. Journal of Biological Chemistry, 289(22), 15413-15425. https://doi.org/10.1074/jbc.M113.543504

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title = "Atypical OmpR/PhoB subfamily response regulator glnr of actinomycetes functions as a homodimer, stabilized by the unphosphorylated conserved Asp-focused charge interactions",

abstract = "Background: Orphan response transcription factor GlnR regulates nitrogen metabolism in important actinomycetes. Results: GlnR has no typical {"}phosphorylation pocket,{"} where the only conserved Asp is unphosphorylated but is essential for functional homodimerization. Conclusion: Actinomycete GlnR is an atypical response regulator functioning as a homodimer. Significance: Conserved Asp-focused charge interactions of actinomycete GlnR are probably the mechanism that stabilizes the homodimer for physiological function.",

author = "Wei Lin and Ying Wang and Xiaobiao Han and Zilong Zhang and Chengyuan Wang and Jin Wang and Huaiyu Yang and Yinhua Lu and Weihong Jiang and Zhao, \{Guo Ping\} and Peng Zhang",

year = "2014",

month = may,

day = "30",

doi = "10.1074/jbc.M113.543504",

language = "英语",

volume = "289",

pages = "15413--15425",

journal = "Journal of Biological Chemistry",

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Lin, W, Wang, Y, Han, X, Zhang, Z, Wang, C, Wang, J, Yang, H, Lu, Y, Jiang, W, Zhao, GP & Zhang, P 2014, 'Atypical OmpR/PhoB subfamily response regulator glnr of actinomycetes functions as a homodimer, stabilized by the unphosphorylated conserved Asp-focused charge interactions', Journal of Biological Chemistry, vol. 289, no. 22, pp. 15413-15425. https://doi.org/10.1074/jbc.M113.543504

TY - JOUR

T1 - Atypical OmpR/PhoB subfamily response regulator glnr of actinomycetes functions as a homodimer, stabilized by the unphosphorylated conserved Asp-focused charge interactions

AU - Lin, Wei

AU - Wang, Ying

AU - Han, Xiaobiao

AU - Zhang, Zilong

AU - Wang, Chengyuan

AU - Wang, Jin

AU - Yang, Huaiyu

AU - Lu, Yinhua

AU - Jiang, Weihong

AU - Zhao, Guo Ping

AU - Zhang, Peng

PY - 2014/5/30

Y1 - 2014/5/30

N2 - Background: Orphan response transcription factor GlnR regulates nitrogen metabolism in important actinomycetes. Results: GlnR has no typical "phosphorylation pocket," where the only conserved Asp is unphosphorylated but is essential for functional homodimerization. Conclusion: Actinomycete GlnR is an atypical response regulator functioning as a homodimer. Significance: Conserved Asp-focused charge interactions of actinomycete GlnR are probably the mechanism that stabilizes the homodimer for physiological function.

AB - Background: Orphan response transcription factor GlnR regulates nitrogen metabolism in important actinomycetes. Results: GlnR has no typical "phosphorylation pocket," where the only conserved Asp is unphosphorylated but is essential for functional homodimerization. Conclusion: Actinomycete GlnR is an atypical response regulator functioning as a homodimer. Significance: Conserved Asp-focused charge interactions of actinomycete GlnR are probably the mechanism that stabilizes the homodimer for physiological function.

UR - https://www.scopus.com/pages/publications/84901724798

U2 - 10.1074/jbc.M113.543504

DO - 10.1074/jbc.M113.543504

M3 - 文章

C2 - 24733389

AN - SCOPUS:84901724798

SN - 0021-9258

VL - 289

SP - 15413

EP - 15425

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 22

ER -

Atypical OmpR/PhoB subfamily response regulator glnr of actinomycetes functions as a homodimer, stabilized by the unphosphorylated conserved Asp-focused charge interactions

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