Assessment of 4-nitro-1,8-naphthalic anhydride reductase activity in homogenates of bakers' yeast by reversed-phase high-performance liquid chromatography

Jianqiang Xu; Qing Yang; Xuhong Qian; Jörgen Samuelsson; Jan Christer Janson

doi:10.1016/j.jchromb.2006.09.040

Assessment of 4-nitro-1,8-naphthalic anhydride reductase activity in homogenates of bakers' yeast by reversed-phase high-performance liquid chromatography

Jianqiang Xu
, Qing Yang
, Xuhong Qian
, Jörgen Samuelsson
, Jan Christer Janson^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method was developed for the simultaneous determination of yield and conversion ratio of 4-nitro-1,8-naphthalic anhydride to 4-amino-1,8-naphthalic anhydride following incubation with a crude bakers' yeast homogenate. The analytes were separated on a C18 column in gradient mode. The detection limit of 4-amino-1,8-naphthalic anhydride is 10 ng/μl when using a 10 μl sample injection volume. The nitroreductase activity in the homogenate system can be assessed during the bioconversion process. The method can be used for the simultaneous detection of 4-hydroxylamino-1,8-naphthalic anhydride, an intermediate with limited stability.

Original language	English
Pages (from-to)	82-87
Number of pages	6
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	847
Issue number	2
DOIs	https://doi.org/10.1016/j.jchromb.2006.09.040
State	Published - 1 Mar 2007
Externally published	Yes

Keywords

Aminoaryl
Arylhydroxylamine
Bakers' yeast
HPLC
Nitroarene
Nitroaryl
Nitroarylreductase
TLC

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10.1016/j.jchromb.2006.09.040

Cite this

Xu, J., Yang, Q., Qian, X., Samuelsson, J., & Janson, J. C. (2007). Assessment of 4-nitro-1,8-naphthalic anhydride reductase activity in homogenates of bakers' yeast by reversed-phase high-performance liquid chromatography. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 847(2), 82-87. https://doi.org/10.1016/j.jchromb.2006.09.040

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title = "Assessment of 4-nitro-1,8-naphthalic anhydride reductase activity in homogenates of bakers' yeast by reversed-phase high-performance liquid chromatography",

abstract = "A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method was developed for the simultaneous determination of yield and conversion ratio of 4-nitro-1,8-naphthalic anhydride to 4-amino-1,8-naphthalic anhydride following incubation with a crude bakers' yeast homogenate. The analytes were separated on a C18 column in gradient mode. The detection limit of 4-amino-1,8-naphthalic anhydride is 10 ng/μl when using a 10 μl sample injection volume. The nitroreductase activity in the homogenate system can be assessed during the bioconversion process. The method can be used for the simultaneous detection of 4-hydroxylamino-1,8-naphthalic anhydride, an intermediate with limited stability.",

keywords = "Aminoaryl, Arylhydroxylamine, Bakers' yeast, HPLC, Nitroarene, Nitroaryl, Nitroarylreductase, TLC",

author = "Jianqiang Xu and Qing Yang and Xuhong Qian and J{\"o}rgen Samuelsson and Janson, \{Jan Christer\}",

year = "2007",

month = mar,

day = "1",

doi = "10.1016/j.jchromb.2006.09.040",

language = "英语",

volume = "847",

pages = "82--87",

journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

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Xu, J, Yang, Q, Qian, X, Samuelsson, J & Janson, JC 2007, 'Assessment of 4-nitro-1,8-naphthalic anhydride reductase activity in homogenates of bakers' yeast by reversed-phase high-performance liquid chromatography', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 847, no. 2, pp. 82-87. https://doi.org/10.1016/j.jchromb.2006.09.040

Assessment of 4-nitro-1,8-naphthalic anhydride reductase activity in homogenates of bakers' yeast by reversed-phase high-performance liquid chromatography. / Xu, Jianqiang; Yang, Qing; Qian, Xuhong et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 847, No. 2, 01.03.2007, p. 82-87.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Assessment of 4-nitro-1,8-naphthalic anhydride reductase activity in homogenates of bakers' yeast by reversed-phase high-performance liquid chromatography

AU - Xu, Jianqiang

AU - Yang, Qing

AU - Qian, Xuhong

AU - Samuelsson, Jörgen

AU - Janson, Jan Christer

PY - 2007/3/1

Y1 - 2007/3/1

N2 - A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method was developed for the simultaneous determination of yield and conversion ratio of 4-nitro-1,8-naphthalic anhydride to 4-amino-1,8-naphthalic anhydride following incubation with a crude bakers' yeast homogenate. The analytes were separated on a C18 column in gradient mode. The detection limit of 4-amino-1,8-naphthalic anhydride is 10 ng/μl when using a 10 μl sample injection volume. The nitroreductase activity in the homogenate system can be assessed during the bioconversion process. The method can be used for the simultaneous detection of 4-hydroxylamino-1,8-naphthalic anhydride, an intermediate with limited stability.

AB - A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method was developed for the simultaneous determination of yield and conversion ratio of 4-nitro-1,8-naphthalic anhydride to 4-amino-1,8-naphthalic anhydride following incubation with a crude bakers' yeast homogenate. The analytes were separated on a C18 column in gradient mode. The detection limit of 4-amino-1,8-naphthalic anhydride is 10 ng/μl when using a 10 μl sample injection volume. The nitroreductase activity in the homogenate system can be assessed during the bioconversion process. The method can be used for the simultaneous detection of 4-hydroxylamino-1,8-naphthalic anhydride, an intermediate with limited stability.

KW - Aminoaryl

KW - Arylhydroxylamine

KW - Bakers' yeast

KW - HPLC

KW - Nitroarene

KW - Nitroaryl

KW - Nitroarylreductase

KW - TLC

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DO - 10.1016/j.jchromb.2006.09.040

M3 - 文章

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AN - SCOPUS:33847286359

SN - 1570-0232

VL - 847

SP - 82

EP - 87

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 2

ER -

Assessment of 4-nitro-1,8-naphthalic anhydride reductase activity in homogenates of bakers' yeast by reversed-phase high-performance liquid chromatography

Abstract

Keywords

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