TY - JOUR
T1 - Antibiotic-resistant bacteria and antibiotic resistance genes in uranium mine
T2 - Distribution and influencing factors
AU - Zhou, Shuai
AU - Xiong, Cong
AU - Su, Yinglong
AU - Wang, Yayi
AU - Gao, Yuanyuan
AU - Tang, Zhenping
AU - Liu, Boyang
AU - Wu, Yueyue
AU - Duan, Yi
N1 - Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2022/7/1
Y1 - 2022/7/1
N2 - Both heavy metals and radiation could affect the proliferation and dissemination of emerging antibiotic resistance pollutants. As an environmental medium rich in radioactive metals, the profile of antibiotic resistance in uranium mine remains largely unknown. A uranium mine in Guangdong province, China was selected to investigate the distribution and influencing factors of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) including intracellular ARGs (iARGs), adsorbed-extracellular ARGs (aeARGs), and free extracellular ARGs (feARGs). The result indicated that sulfonamide and tetracycline ARB could be generally detected in mining area with the absolute concentrations of 7.70 × 102–5.18 × 105 colony forming unit/g. The abundances of aeARGs in mine soil were significantly higher than those of iARGs (p < 0.05), highlighting the critical contribution of aeARGs to ARGs spread. The feARGs in mine drainage and its receiving river were abundant (3.38 × 104–1.86 × 107 copies/mL). ARB, aeARGs, and iARGs may correlate with nitrogen species and heavy metals (e.g., U and Mn), and feARGs presented a significant correlation with chemical oxygen demand (p < 0.05). These findings demonstrate the occurrence of ARB and ARGs in uranium mine for the first time, thereby contributing to the assessment and control of the ecological risk of antibiotic resistance in radioactive environments.
AB - Both heavy metals and radiation could affect the proliferation and dissemination of emerging antibiotic resistance pollutants. As an environmental medium rich in radioactive metals, the profile of antibiotic resistance in uranium mine remains largely unknown. A uranium mine in Guangdong province, China was selected to investigate the distribution and influencing factors of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) including intracellular ARGs (iARGs), adsorbed-extracellular ARGs (aeARGs), and free extracellular ARGs (feARGs). The result indicated that sulfonamide and tetracycline ARB could be generally detected in mining area with the absolute concentrations of 7.70 × 102–5.18 × 105 colony forming unit/g. The abundances of aeARGs in mine soil were significantly higher than those of iARGs (p < 0.05), highlighting the critical contribution of aeARGs to ARGs spread. The feARGs in mine drainage and its receiving river were abundant (3.38 × 104–1.86 × 107 copies/mL). ARB, aeARGs, and iARGs may correlate with nitrogen species and heavy metals (e.g., U and Mn), and feARGs presented a significant correlation with chemical oxygen demand (p < 0.05). These findings demonstrate the occurrence of ARB and ARGs in uranium mine for the first time, thereby contributing to the assessment and control of the ecological risk of antibiotic resistance in radioactive environments.
KW - Antibiotic resistance genes (ARGs)
KW - Antibiotic-resistant bacteria (ARB)
KW - Distribution
KW - Influencing factor
KW - Uranium mine
UR - https://www.scopus.com/pages/publications/85126975073
U2 - 10.1016/j.envpol.2022.119158
DO - 10.1016/j.envpol.2022.119158
M3 - 文章
C2 - 35304179
AN - SCOPUS:85126975073
SN - 0269-7491
VL - 304
JO - Environmental Pollution
JF - Environmental Pollution
M1 - 119158
ER -