Anti-lipid peroxidation of gomisin J on liver mitochondria and cultured myocardial cells

Hong Li Peng; Dao Feng Chen; Hong Xiang Lan; Xue Mei Zhang; Zheng Gu; Ming Hua Jiang

Anti-lipid peroxidation of gomisin J on liver mitochondria and cultured myocardial cells

Hong Li Peng, Dao Feng Chen, Hong Xiang Lan, Xue Mei Zhang, Zheng Gu, Ming Hua Jiang

Fudan University

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Aim: To study the influences of gomisin J on lipid peroxidation and calcium paradox. Methods: Using two in vitro models of rat liver mitochondria membrane lipid peroxidation (LPO) and cultured myocardial cells. Results: Gomisin J inhibited Fe²⁺/ascorbic acid and ADP/NADPH-induced LPO with IC₅₀ (95% confidence limits) 5.5 (4.5-6.7) and 4.7 (2.8-7.8) μmol·L^-1, respectively, when cultured myocardial cells preincubated with Ca²⁺-free medium for 2 min were incubated with normal medium containing Ca²⁺, a marked increase of malondialdehyde (MDA) formation occurred and gomisin J 10 μmol·L^-1 protected myocardial cells through decreasing MDA formation. Conclusion: Gomisin J inhibits LPO in rat liver mitochondria and protects cultured myocardial cells from being injuried by calcium paradox.

Original language	English
Pages (from-to)	538-541
Number of pages	4
Journal	Acta Pharmacologica Sinica
Volume	17
Issue number	6
State	Published - Nov 1996
Externally published	Yes

Keywords

cultured cells
gomisin J
lignans
lipid peroxidation
liver mitochondria
myocardium

Cite this

@article{7947563c6fe04d9ea1f6bab2a53b4b5c,

title = "Anti-lipid peroxidation of gomisin J on liver mitochondria and cultured myocardial cells",

abstract = "Aim: To study the influences of gomisin J on lipid peroxidation and calcium paradox. Methods: Using two in vitro models of rat liver mitochondria membrane lipid peroxidation (LPO) and cultured myocardial cells. Results: Gomisin J inhibited Fe2+/ascorbic acid and ADP/NADPH-induced LPO with IC50 (95\% confidence limits) 5.5 (4.5-6.7) and 4.7 (2.8-7.8) μmol·L-1, respectively, when cultured myocardial cells preincubated with Ca2+-free medium for 2 min were incubated with normal medium containing Ca2+, a marked increase of malondialdehyde (MDA) formation occurred and gomisin J 10 μmol·L-1 protected myocardial cells through decreasing MDA formation. Conclusion: Gomisin J inhibits LPO in rat liver mitochondria and protects cultured myocardial cells from being injuried by calcium paradox.",

keywords = "cultured cells, gomisin J, lignans, lipid peroxidation, liver mitochondria, myocardium",

author = "Peng, \{Hong Li\} and Chen, \{Dao Feng\} and Lan, \{Hong Xiang\} and Zhang, \{Xue Mei\} and Zheng Gu and Jiang, \{Ming Hua\}",

year = "1996",

month = nov,

language = "英语",

volume = "17",

pages = "538--541",

journal = "Acta Pharmacologica Sinica",

issn = "0253-9756",

publisher = "Springer Nature",

number = "6",

}

TY - JOUR

T1 - Anti-lipid peroxidation of gomisin J on liver mitochondria and cultured myocardial cells

AU - Peng, Hong Li

AU - Chen, Dao Feng

AU - Lan, Hong Xiang

AU - Zhang, Xue Mei

AU - Gu, Zheng

AU - Jiang, Ming Hua

PY - 1996/11

Y1 - 1996/11

N2 - Aim: To study the influences of gomisin J on lipid peroxidation and calcium paradox. Methods: Using two in vitro models of rat liver mitochondria membrane lipid peroxidation (LPO) and cultured myocardial cells. Results: Gomisin J inhibited Fe2+/ascorbic acid and ADP/NADPH-induced LPO with IC50 (95% confidence limits) 5.5 (4.5-6.7) and 4.7 (2.8-7.8) μmol·L-1, respectively, when cultured myocardial cells preincubated with Ca2+-free medium for 2 min were incubated with normal medium containing Ca2+, a marked increase of malondialdehyde (MDA) formation occurred and gomisin J 10 μmol·L-1 protected myocardial cells through decreasing MDA formation. Conclusion: Gomisin J inhibits LPO in rat liver mitochondria and protects cultured myocardial cells from being injuried by calcium paradox.

AB - Aim: To study the influences of gomisin J on lipid peroxidation and calcium paradox. Methods: Using two in vitro models of rat liver mitochondria membrane lipid peroxidation (LPO) and cultured myocardial cells. Results: Gomisin J inhibited Fe2+/ascorbic acid and ADP/NADPH-induced LPO with IC50 (95% confidence limits) 5.5 (4.5-6.7) and 4.7 (2.8-7.8) μmol·L-1, respectively, when cultured myocardial cells preincubated with Ca2+-free medium for 2 min were incubated with normal medium containing Ca2+, a marked increase of malondialdehyde (MDA) formation occurred and gomisin J 10 μmol·L-1 protected myocardial cells through decreasing MDA formation. Conclusion: Gomisin J inhibits LPO in rat liver mitochondria and protects cultured myocardial cells from being injuried by calcium paradox.

KW - cultured cells

KW - gomisin J

KW - lignans

KW - lipid peroxidation

KW - liver mitochondria

KW - myocardium

UR - https://www.scopus.com/pages/publications/0029846304

M3 - 文章

C2 - 9863151

AN - SCOPUS:0029846304

SN - 0253-9756

VL - 17

SP - 538

EP - 541

JO - Acta Pharmacologica Sinica

JF - Acta Pharmacologica Sinica

IS - 6

ER -

Anti-lipid peroxidation of gomisin J on liver mitochondria and cultured myocardial cells

Abstract

Keywords

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