TY - JOUR
T1 - Analysis of neutral sugars of asparagus officinalis linn. Polysaccharide by CZE with amperometric detection
AU - Yang, Zhiyong
AU - Wang, Huan
AU - Zhang, Wen
AU - Wang, Qingjiang
AU - He, Pingang
AU - Fang, Yuzhi
PY - 2012/3
Y1 - 2012/3
N2 - Analysis of the neutral sugars of Asparagus officinalis Linn. polysaccharide by different methods has yielded inconsistent results. In the work discussed in this paper, capillary zone electrophoresis with amperometric detection (CZE-AD) was used for analysis of neutral sugars in A. officinalis Linn. polysaccharide. The configuration of the wall-jet and the diameter of the copper disk electrode were investigated to achieve optimized detection sensitivity. The separation electrolyte, separation voltage, and injection time were studied for their effects on CZE separation. Under the optimum CZE-AD conditions, seven monosaccharides were separated to baseline by using 120 mM NaOH as separation electrolyte. Linear response was excellent and repeatability was satisfactory. It was found that Asparagus polysaccharide was composed of fucose, galactose, glucose, rhamnose, arabinose, fructose, and xylose at a mole ratio of 0.2:16.2:5.0:1.0:15.5:0.6:18.8. Compared with other methods, analysis of the composition of Asparagus polysaccharide by CZE-AD had the merits of rapidness, accuracy, and lower sampling volume.
AB - Analysis of the neutral sugars of Asparagus officinalis Linn. polysaccharide by different methods has yielded inconsistent results. In the work discussed in this paper, capillary zone electrophoresis with amperometric detection (CZE-AD) was used for analysis of neutral sugars in A. officinalis Linn. polysaccharide. The configuration of the wall-jet and the diameter of the copper disk electrode were investigated to achieve optimized detection sensitivity. The separation electrolyte, separation voltage, and injection time were studied for their effects on CZE separation. Under the optimum CZE-AD conditions, seven monosaccharides were separated to baseline by using 120 mM NaOH as separation electrolyte. Linear response was excellent and repeatability was satisfactory. It was found that Asparagus polysaccharide was composed of fucose, galactose, glucose, rhamnose, arabinose, fructose, and xylose at a mole ratio of 0.2:16.2:5.0:1.0:15.5:0.6:18.8. Compared with other methods, analysis of the composition of Asparagus polysaccharide by CZE-AD had the merits of rapidness, accuracy, and lower sampling volume.
KW - Amperometric detection
KW - Analysis of neutral sugars
KW - Asparagus officinalis Linn. polysaccharide
KW - Capillary zone electrophoresis
UR - https://www.scopus.com/pages/publications/84862892191
U2 - 10.1007/s10337-012-2197-5
DO - 10.1007/s10337-012-2197-5
M3 - 文章
AN - SCOPUS:84862892191
SN - 0009-5893
VL - 75
SP - 297
EP - 304
JO - Chromatographia
JF - Chromatographia
IS - 5-6
ER -