Anaerobic ammonium oxidation-denitrification synergistic interaction of mature landfill leachate in aged refuse bioreactor: Variations and effects of microbial community structures

Huawei Wei; Jie Wang; Muhammad Hassan; Lu Han; Bing Xie

doi:10.1016/j.biortech.2017.07.077

Anaerobic ammonium oxidation-denitrification synergistic interaction of mature landfill leachate in aged refuse bioreactor: Variations and effects of microbial community structures

Huawei Wei
, Jie Wang
, Muhammad Hassan
, Lu Han
, Bing Xie^*

^*Corresponding author for this work

School of Ecology and Environmental Sciences

East China Normal University

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

In this work, anammox-denitrification process was verified by ¹⁵N stable isotopic tracing methods and variations and effects of microbial community structures were studied using Illumina MiSeq sequencing and Quantitative Polymerase Chain Reaction (qPCR). The results showed that higher nitrogen removal efficiency and richer microbial consortia was observed at hydraulic loading rate (HLR) of 15 L/m³·d, BOD₅/TN ratio of 0.4:1, respectively. Proteobacteria, Chloroflexi, Acidobacteria and Firmicutes were the dominant phyla in the anamox-denitrification biomass. The number of amx gene changed significantly during the HLR downshift and BOD₅/TN ratio upshift period. The obtained results enhance understanding regarding the microbial community structures of anammox-denitrification bacteria in aged refuse, leading to a more effective controlling of anammox-denitrification process.

Original language	English
Pages (from-to)	1149-1158
Number of pages	10
Journal	Bioresource Technology
Volume	243
DOIs	https://doi.org/10.1016/j.biortech.2017.07.077
State	Published - 2017

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy

Keywords

Aged refuse bioreactor
Anammox
Denitrification
Hydraulic loading rate
Microbial community structures

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10.1016/j.biortech.2017.07.077

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title = "Anaerobic ammonium oxidation-denitrification synergistic interaction of mature landfill leachate in aged refuse bioreactor: Variations and effects of microbial community structures",

abstract = "In this work, anammox-denitrification process was verified by 15N stable isotopic tracing methods and variations and effects of microbial community structures were studied using Illumina MiSeq sequencing and Quantitative Polymerase Chain Reaction (qPCR). The results showed that higher nitrogen removal efficiency and richer microbial consortia was observed at hydraulic loading rate (HLR) of 15 L/m3·d, BOD5/TN ratio of 0.4:1, respectively. Proteobacteria, Chloroflexi, Acidobacteria and Firmicutes were the dominant phyla in the anamox-denitrification biomass. The number of amx gene changed significantly during the HLR downshift and BOD5/TN ratio upshift period. The obtained results enhance understanding regarding the microbial community structures of anammox-denitrification bacteria in aged refuse, leading to a more effective controlling of anammox-denitrification process.",

keywords = "Aged refuse bioreactor, Anammox, Denitrification, Hydraulic loading rate, Microbial community structures",

author = "Huawei Wei and Jie Wang and Muhammad Hassan and Lu Han and Bing Xie",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

year = "2017",

doi = "10.1016/j.biortech.2017.07.077",

language = "英语",

volume = "243",

pages = "1149--1158",

journal = "Bioresource Technology",

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T1 - Anaerobic ammonium oxidation-denitrification synergistic interaction of mature landfill leachate in aged refuse bioreactor

T2 - Variations and effects of microbial community structures

AU - Wei, Huawei

AU - Wang, Jie

AU - Hassan, Muhammad

AU - Han, Lu

AU - Xie, Bing

PY - 2017

Y1 - 2017

N2 - In this work, anammox-denitrification process was verified by 15N stable isotopic tracing methods and variations and effects of microbial community structures were studied using Illumina MiSeq sequencing and Quantitative Polymerase Chain Reaction (qPCR). The results showed that higher nitrogen removal efficiency and richer microbial consortia was observed at hydraulic loading rate (HLR) of 15 L/m3·d, BOD5/TN ratio of 0.4:1, respectively. Proteobacteria, Chloroflexi, Acidobacteria and Firmicutes were the dominant phyla in the anamox-denitrification biomass. The number of amx gene changed significantly during the HLR downshift and BOD5/TN ratio upshift period. The obtained results enhance understanding regarding the microbial community structures of anammox-denitrification bacteria in aged refuse, leading to a more effective controlling of anammox-denitrification process.

AB - In this work, anammox-denitrification process was verified by 15N stable isotopic tracing methods and variations and effects of microbial community structures were studied using Illumina MiSeq sequencing and Quantitative Polymerase Chain Reaction (qPCR). The results showed that higher nitrogen removal efficiency and richer microbial consortia was observed at hydraulic loading rate (HLR) of 15 L/m3·d, BOD5/TN ratio of 0.4:1, respectively. Proteobacteria, Chloroflexi, Acidobacteria and Firmicutes were the dominant phyla in the anamox-denitrification biomass. The number of amx gene changed significantly during the HLR downshift and BOD5/TN ratio upshift period. The obtained results enhance understanding regarding the microbial community structures of anammox-denitrification bacteria in aged refuse, leading to a more effective controlling of anammox-denitrification process.

KW - Aged refuse bioreactor

KW - Anammox

KW - Denitrification

KW - Hydraulic loading rate

KW - Microbial community structures

UR - https://www.scopus.com/pages/publications/85026431588

U2 - 10.1016/j.biortech.2017.07.077

DO - 10.1016/j.biortech.2017.07.077

M3 - 文章

C2 - 28764129

AN - SCOPUS:85026431588

SN - 0960-8524

VL - 243

SP - 1149

EP - 1158

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Anaerobic ammonium oxidation-denitrification synergistic interaction of mature landfill leachate in aged refuse bioreactor: Variations and effects of microbial community structures

Abstract

UN SDGs

Keywords

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