An unnatural amino acid based fluorescent probe for phenylalanine ammonia lyase

Zhenlin Tian; Weiping Zhu; Yufang Xu; Xuhong Qian

doi:10.1039/c4ob00914b

An unnatural amino acid based fluorescent probe for phenylalanine ammonia lyase

Zhenlin Tian
, Weiping Zhu^*
, Yufang Xu
, Xuhong Qian

^*Corresponding author for this work

East China University of Science and Technology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A fluorescent probe (2a-LP) based on an unnatural amino acid (UAA) is developed for the detection of phenylalanine ammonia lyase (PAL). In the presence of PAL, 2a-LP is catalytically deaminated to ortho-amino-transcinnamic acid (o-a-CA), which shows a remarkable "off-on" fluorescence signal. Thus, the probe 2a-LP enables direct visualization of the PAL activity in tomato under UV illumination and has potential in vitro assays. This journal is

Original language	English
Pages (from-to)	5818-5821
Number of pages	4
Journal	Organic and Biomolecular Chemistry
Volume	12
Issue number	31
DOIs	https://doi.org/10.1039/c4ob00914b
State	Published - 21 Aug 2014
Externally published	Yes

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AU - Tian, Zhenlin

AU - Zhu, Weiping

AU - Xu, Yufang

AU - Qian, Xuhong

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Y1 - 2014/8/21

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AB - A fluorescent probe (2a-LP) based on an unnatural amino acid (UAA) is developed for the detection of phenylalanine ammonia lyase (PAL). In the presence of PAL, 2a-LP is catalytically deaminated to ortho-amino-transcinnamic acid (o-a-CA), which shows a remarkable "off-on" fluorescence signal. Thus, the probe 2a-LP enables direct visualization of the PAL activity in tomato under UV illumination and has potential in vitro assays. This journal is

UR - https://www.scopus.com/pages/publications/84904410911

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DO - 10.1039/c4ob00914b

M3 - 文章

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AN - SCOPUS:84904410911

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SP - 5818

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JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

IS - 31

ER -

An unnatural amino acid based fluorescent probe for phenylalanine ammonia lyase

Abstract

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