An Atlas of Thyroid Hormone Responsive Genes in Adult Mouse Hypothalamus

Shijia Wu; Julien Dellinger; Suzy Markossian; Yves Dusabyinema; Romain Guyot; Sandrine Hughes; Denise Aubert; Marie Fackeure; Karine Gauthier; Benjamin Gillet; Wenzheng Jiang; Frédéric Flamant

doi:10.1210/endocr/bqaf084

An Atlas of Thyroid Hormone Responsive Genes in Adult Mouse Hypothalamus

Shijia Wu, Julien Dellinger, Suzy Markossian, Yves Dusabyinema, Romain Guyot, Sandrine Hughes, Denise Aubert, Marie Fackeure, Karine Gauthier, Benjamin Gillet, Wenzheng Jiang, Frédéric Flamant

School of Life Sciences

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We present an atlas of genes that respond to thyroid hormone in the adult mouse mediobasal hypothalamus. Based on droplet-based single nuclei RNA-seq method and batch transcriptome analyses, the atlas lists putative target genes of the hormone nuclear receptors in 20 different types of neuronal and glial cells. The transcriptional regulation of these genes varies extensively across neuronal and glial cell types. However, while astrocytes appear to be highly sensitive to thyroid hormone stimulation, differentiated oligodendrocytes are relatively insensitive. This atlas is expected to promote future investigations into the molecular and cellular mechanisms that underlie the numerous functions of thyroid hormone in the hypothalamic circuits.

Original language	English
Article number	bqaf084
Journal	Endocrinology
Volume	166
Issue number	6
DOIs	https://doi.org/10.1210/endocr/bqaf084
State	Published - 1 Jun 2025

Keywords

bulk RNA-seq
hypothalamus
single nuclei RNA-seq
single nuclei analysis
thyroid hormone

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10.1210/endocr/bqaf084

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abstract = "We present an atlas of genes that respond to thyroid hormone in the adult mouse mediobasal hypothalamus. Based on droplet-based single nuclei RNA-seq method and batch transcriptome analyses, the atlas lists putative target genes of the hormone nuclear receptors in 20 different types of neuronal and glial cells. The transcriptional regulation of these genes varies extensively across neuronal and glial cell types. However, while astrocytes appear to be highly sensitive to thyroid hormone stimulation, differentiated oligodendrocytes are relatively insensitive. This atlas is expected to promote future investigations into the molecular and cellular mechanisms that underlie the numerous functions of thyroid hormone in the hypothalamic circuits.",

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AU - Wu, Shijia

AU - Dellinger, Julien

AU - Markossian, Suzy

AU - Dusabyinema, Yves

AU - Guyot, Romain

AU - Hughes, Sandrine

AU - Aubert, Denise

AU - Fackeure, Marie

AU - Gauthier, Karine

AU - Gillet, Benjamin

AU - Jiang, Wenzheng

AU - Flamant, Frédéric

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N2 - We present an atlas of genes that respond to thyroid hormone in the adult mouse mediobasal hypothalamus. Based on droplet-based single nuclei RNA-seq method and batch transcriptome analyses, the atlas lists putative target genes of the hormone nuclear receptors in 20 different types of neuronal and glial cells. The transcriptional regulation of these genes varies extensively across neuronal and glial cell types. However, while astrocytes appear to be highly sensitive to thyroid hormone stimulation, differentiated oligodendrocytes are relatively insensitive. This atlas is expected to promote future investigations into the molecular and cellular mechanisms that underlie the numerous functions of thyroid hormone in the hypothalamic circuits.

AB - We present an atlas of genes that respond to thyroid hormone in the adult mouse mediobasal hypothalamus. Based on droplet-based single nuclei RNA-seq method and batch transcriptome analyses, the atlas lists putative target genes of the hormone nuclear receptors in 20 different types of neuronal and glial cells. The transcriptional regulation of these genes varies extensively across neuronal and glial cell types. However, while astrocytes appear to be highly sensitive to thyroid hormone stimulation, differentiated oligodendrocytes are relatively insensitive. This atlas is expected to promote future investigations into the molecular and cellular mechanisms that underlie the numerous functions of thyroid hormone in the hypothalamic circuits.

KW - bulk RNA-seq

KW - hypothalamus

KW - single nuclei RNA-seq

KW - single nuclei analysis

KW - thyroid hormone

UR - https://www.scopus.com/pages/publications/105005069194

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An Atlas of Thyroid Hormone Responsive Genes in Adult Mouse Hypothalamus

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