An aqueous platinum nanotube based fluorescent immuno-assay for porcine reproductive and respiratory syndrome virus detection

Lu Chen; Shiyi Ye; Kai Cai; Cuiling Zhang; Guohua Zhou; Zhike He; Heyou Han

doi:10.1016/j.talanta.2015.06.061

An aqueous platinum nanotube based fluorescent immuno-assay for porcine reproductive and respiratory syndrome virus detection

Lu Chen
, Shiyi Ye
, Kai Cai
, Cuiling Zhang
, Guohua Zhou
, Zhike He
, Heyou Han^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) has been a significant pathogen towards global swine industry upon its emergence in the late 1980s and since then has exemplified a rapidly evolving, widely spreading pathogen. It is urgently important to develop a simple, rapid and cost effective method to detect this pathogen when virus outbreaks. In the present work, it was found that virus antibody modified platinum nanotubes (Pt-Ab) could act as a superquencher to CdTe:Zn²⁺ quantum dots (CdTe:Zn²⁺ QDs) fluorescence by Stern-Volmer constants nearly 10⁹ M^-1 without any aggregation, the CdTe:Zn²⁺ QDs fluorescence will recover as the Pt-Ab goes away by antibody and antigen interaction when virus was added into the probe solution, releasing CdTe:Zn²⁺ QDs from the surface of Pt-Ab. By the recovery fluorescence intensity, it can realize qualitative and quantitative detection of PRRSV. This method gives a fast response to PRRSV concentration and provides a sensitive detection limit (2.4 ng/mL). Moreover, it can be applied in infected porcine serum samples and obtain satisfied results.

Original language	English
Pages (from-to)	324-328
Number of pages	5
Journal	Talanta
Volume	144
DOIs	https://doi.org/10.1016/j.talanta.2015.06.061
State	Published - 4 Jul 2015
Externally published	Yes

Keywords

Fluorescence
PRRSV detection
PtNTs
Quantum dot

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10.1016/j.talanta.2015.06.061

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@article{224e1d7375224ae5bbe3bf95bb31897f,

title = "An aqueous platinum nanotube based fluorescent immuno-assay for porcine reproductive and respiratory syndrome virus detection",

abstract = "Porcine reproductive and respiratory syndrome virus (PRRSV) has been a significant pathogen towards global swine industry upon its emergence in the late 1980s and since then has exemplified a rapidly evolving, widely spreading pathogen. It is urgently important to develop a simple, rapid and cost effective method to detect this pathogen when virus outbreaks. In the present work, it was found that virus antibody modified platinum nanotubes (Pt-Ab) could act as a superquencher to CdTe:Zn2+ quantum dots (CdTe:Zn2+ QDs) fluorescence by Stern-Volmer constants nearly 109 M-1 without any aggregation, the CdTe:Zn2+ QDs fluorescence will recover as the Pt-Ab goes away by antibody and antigen interaction when virus was added into the probe solution, releasing CdTe:Zn2+ QDs from the surface of Pt-Ab. By the recovery fluorescence intensity, it can realize qualitative and quantitative detection of PRRSV. This method gives a fast response to PRRSV concentration and provides a sensitive detection limit (2.4 ng/mL). Moreover, it can be applied in infected porcine serum samples and obtain satisfied results.",

keywords = "Fluorescence, PRRSV detection, PtNTs, Quantum dot",

author = "Lu Chen and Shiyi Ye and Kai Cai and Cuiling Zhang and Guohua Zhou and Zhike He and Heyou Han",

year = "2015",

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day = "4",

doi = "10.1016/j.talanta.2015.06.061",

language = "英语",

volume = "144",

pages = "324--328",

journal = "Talanta",

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T1 - An aqueous platinum nanotube based fluorescent immuno-assay for porcine reproductive and respiratory syndrome virus detection

AU - Chen, Lu

AU - Ye, Shiyi

AU - Cai, Kai

AU - Zhang, Cuiling

AU - Zhou, Guohua

AU - He, Zhike

AU - Han, Heyou

PY - 2015/7/4

Y1 - 2015/7/4

N2 - Porcine reproductive and respiratory syndrome virus (PRRSV) has been a significant pathogen towards global swine industry upon its emergence in the late 1980s and since then has exemplified a rapidly evolving, widely spreading pathogen. It is urgently important to develop a simple, rapid and cost effective method to detect this pathogen when virus outbreaks. In the present work, it was found that virus antibody modified platinum nanotubes (Pt-Ab) could act as a superquencher to CdTe:Zn2+ quantum dots (CdTe:Zn2+ QDs) fluorescence by Stern-Volmer constants nearly 109 M-1 without any aggregation, the CdTe:Zn2+ QDs fluorescence will recover as the Pt-Ab goes away by antibody and antigen interaction when virus was added into the probe solution, releasing CdTe:Zn2+ QDs from the surface of Pt-Ab. By the recovery fluorescence intensity, it can realize qualitative and quantitative detection of PRRSV. This method gives a fast response to PRRSV concentration and provides a sensitive detection limit (2.4 ng/mL). Moreover, it can be applied in infected porcine serum samples and obtain satisfied results.

AB - Porcine reproductive and respiratory syndrome virus (PRRSV) has been a significant pathogen towards global swine industry upon its emergence in the late 1980s and since then has exemplified a rapidly evolving, widely spreading pathogen. It is urgently important to develop a simple, rapid and cost effective method to detect this pathogen when virus outbreaks. In the present work, it was found that virus antibody modified platinum nanotubes (Pt-Ab) could act as a superquencher to CdTe:Zn2+ quantum dots (CdTe:Zn2+ QDs) fluorescence by Stern-Volmer constants nearly 109 M-1 without any aggregation, the CdTe:Zn2+ QDs fluorescence will recover as the Pt-Ab goes away by antibody and antigen interaction when virus was added into the probe solution, releasing CdTe:Zn2+ QDs from the surface of Pt-Ab. By the recovery fluorescence intensity, it can realize qualitative and quantitative detection of PRRSV. This method gives a fast response to PRRSV concentration and provides a sensitive detection limit (2.4 ng/mL). Moreover, it can be applied in infected porcine serum samples and obtain satisfied results.

KW - Fluorescence

KW - PRRSV detection

KW - PtNTs

KW - Quantum dot

UR - https://www.scopus.com/pages/publications/84947288704

U2 - 10.1016/j.talanta.2015.06.061

DO - 10.1016/j.talanta.2015.06.061

M3 - 文章

C2 - 26452829

AN - SCOPUS:84947288704

SN - 0039-9140

VL - 144

SP - 324

EP - 328

JO - Talanta

JF - Talanta

ER -

An aqueous platinum nanotube based fluorescent immuno-assay for porcine reproductive and respiratory syndrome virus detection

Abstract

Keywords

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