TY - GEN
T1 - An AOTF-based molecular hyperspectral imaging (MHSI) system
AU - Liu, Hongying
AU - Li, Qingli
AU - Guan, Yana
AU - Liu, Jingao
AU - Xue, Yongqi
PY - 2011
Y1 - 2011
N2 - A novel molecular hyperspectral imaging (MHSI) system based on AOTF (acousto-optic tunable filters) was presented. AOTF is a rapid wavelength-scanning solid-state device that operates as a tunable optical band pass filter. AOTF offers the advantage of having no moving parts and can be scanned at very high rates. The system consists of microscope, AOTF-based spectrometer, matrix CCD, image collection card and computer. The spectral range of the MHSI is from 550 to 1000 nm. The spectral resolution is less than 2 nm, and the spatial resolution is about 0.3 μm. The valid pixels are 1024×1024. The system can capture 30 frames per min in burst mode of CCD. The system not only can supply single band images in the visible range, but also spectrum curve of random pixel of sample image. Each pixel of the image has two properties: the spectral property and the luminance property. Initial experiment shows that molecular hyperspectral imaging system coupled with multivariate data analysis is a powerful new tool for understanding complex biological and biomedical samples.
AB - A novel molecular hyperspectral imaging (MHSI) system based on AOTF (acousto-optic tunable filters) was presented. AOTF is a rapid wavelength-scanning solid-state device that operates as a tunable optical band pass filter. AOTF offers the advantage of having no moving parts and can be scanned at very high rates. The system consists of microscope, AOTF-based spectrometer, matrix CCD, image collection card and computer. The spectral range of the MHSI is from 550 to 1000 nm. The spectral resolution is less than 2 nm, and the spatial resolution is about 0.3 μm. The valid pixels are 1024×1024. The system can capture 30 frames per min in burst mode of CCD. The system not only can supply single band images in the visible range, but also spectrum curve of random pixel of sample image. Each pixel of the image has two properties: the spectral property and the luminance property. Initial experiment shows that molecular hyperspectral imaging system coupled with multivariate data analysis is a powerful new tool for understanding complex biological and biomedical samples.
KW - Acoustooptic tunable filters
KW - Biochemical detection
KW - CCD
KW - Molecular hyperspctral imaging(MHSI)
UR - https://www.scopus.com/pages/publications/79960116886
U2 - 10.1109/icbbe.2011.5780363
DO - 10.1109/icbbe.2011.5780363
M3 - 会议稿件
AN - SCOPUS:79960116886
SN - 9781424450893
T3 - 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011
BT - 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011
T2 - 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011
Y2 - 10 May 2011 through 12 May 2011
ER -