Amperometric sensor based on neutral red-doped silica nanoparticles coupled with microdialysis for the measurement of glutamate in the rat striatum

Xiao Hua Li; Yue Zhong Xian; Zong Hong Xie; Hong Min; Chen Xin Li; Li Tong Jin

doi:10.1002/cjoc.200790185

Amperometric sensor based on neutral red-doped silica nanoparticles coupled with microdialysis for the measurement of glutamate in the rat striatum

Xiao Hua Li, Yue Zhong Xian, Zong Hong Xie, Hong Min, Chen Xin Li, Li Tong Jin

School of Chemistry and Molecular Engineering

East China Normal University

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Amperometric sensor based on neutral red-doped silica (NRSiO₂) nanoparticles (NPs) was fabricated and coupled with a microdialysis sampling system for the detection of glutamate (Glu) in the rat striatum. The NRSiO ₂ NPs [about (45 ± 3) nm] were prepared with water-in-oil (W/O) microemulsion method, and characterized by transmission electron microscope (TEM) technique. The neutral red (NR) doped in silica network could maintain its high electroactivity and behave as an excellent electron mediator for electrocatalysis of hydrogen dioxide. Furthermore, the silica surface could prevent the leakage of NR, hence, the stability of biosensor was improved. The novel Glu biosensor showed a linear range from 5.0 × 10^-7 to 1.5 × 10^-4 mol/L, with a detection limit of 2.0 × 10 ^-7 mol/L (S/N=3).

Original language	English
Pages (from-to)	953-957
Number of pages	5
Journal	Chinese Journal of Chemistry
Volume	25
Issue number	7
DOIs	https://doi.org/10.1002/cjoc.200790185
State	Published - Jul 2007

Keywords

Glutamate biosensor
Glutamate oxidase (GluOx)
Microdialysis
NRSiO
Rat striatum

Access to Document

10.1002/cjoc.200790185

Cite this

@article{18828a78752344748ad66390a5324087,

title = "Amperometric sensor based on neutral red-doped silica nanoparticles coupled with microdialysis for the measurement of glutamate in the rat striatum",

abstract = "Amperometric sensor based on neutral red-doped silica (NRSiO2) nanoparticles (NPs) was fabricated and coupled with a microdialysis sampling system for the detection of glutamate (Glu) in the rat striatum. The NRSiO 2 NPs [about (45 ± 3) nm] were prepared with water-in-oil (W/O) microemulsion method, and characterized by transmission electron microscope (TEM) technique. The neutral red (NR) doped in silica network could maintain its high electroactivity and behave as an excellent electron mediator for electrocatalysis of hydrogen dioxide. Furthermore, the silica surface could prevent the leakage of NR, hence, the stability of biosensor was improved. The novel Glu biosensor showed a linear range from 5.0 × 10-7 to 1.5 × 10-4 mol/L, with a detection limit of 2.0 × 10 -7 mol/L (S/N=3).",

keywords = "Glutamate biosensor, Glutamate oxidase (GluOx), Microdialysis, NRSiO, Rat striatum",

author = "Li, \{Xiao Hua\} and Xian, \{Yue Zhong\} and Xie, \{Zong Hong\} and Hong Min and Li, \{Chen Xin\} and Jin, \{Li Tong\}",

year = "2007",

month = jul,

doi = "10.1002/cjoc.200790185",

language = "英语",

volume = "25",

pages = "953--957",

journal = "Chinese Journal of Chemistry",

issn = "1001-604X",

publisher = "John Wiley and Sons Inc",

number = "7",

}

TY - JOUR

T1 - Amperometric sensor based on neutral red-doped silica nanoparticles coupled with microdialysis for the measurement of glutamate in the rat striatum

AU - Li, Xiao Hua

AU - Xian, Yue Zhong

AU - Xie, Zong Hong

AU - Min, Hong

AU - Li, Chen Xin

AU - Jin, Li Tong

PY - 2007/7

Y1 - 2007/7

N2 - Amperometric sensor based on neutral red-doped silica (NRSiO2) nanoparticles (NPs) was fabricated and coupled with a microdialysis sampling system for the detection of glutamate (Glu) in the rat striatum. The NRSiO 2 NPs [about (45 ± 3) nm] were prepared with water-in-oil (W/O) microemulsion method, and characterized by transmission electron microscope (TEM) technique. The neutral red (NR) doped in silica network could maintain its high electroactivity and behave as an excellent electron mediator for electrocatalysis of hydrogen dioxide. Furthermore, the silica surface could prevent the leakage of NR, hence, the stability of biosensor was improved. The novel Glu biosensor showed a linear range from 5.0 × 10-7 to 1.5 × 10-4 mol/L, with a detection limit of 2.0 × 10 -7 mol/L (S/N=3).

AB - Amperometric sensor based on neutral red-doped silica (NRSiO2) nanoparticles (NPs) was fabricated and coupled with a microdialysis sampling system for the detection of glutamate (Glu) in the rat striatum. The NRSiO 2 NPs [about (45 ± 3) nm] were prepared with water-in-oil (W/O) microemulsion method, and characterized by transmission electron microscope (TEM) technique. The neutral red (NR) doped in silica network could maintain its high electroactivity and behave as an excellent electron mediator for electrocatalysis of hydrogen dioxide. Furthermore, the silica surface could prevent the leakage of NR, hence, the stability of biosensor was improved. The novel Glu biosensor showed a linear range from 5.0 × 10-7 to 1.5 × 10-4 mol/L, with a detection limit of 2.0 × 10 -7 mol/L (S/N=3).

KW - Glutamate biosensor

KW - Glutamate oxidase (GluOx)

KW - Microdialysis

KW - NRSiO

KW - Rat striatum

UR - https://www.scopus.com/pages/publications/34548106897

U2 - 10.1002/cjoc.200790185

DO - 10.1002/cjoc.200790185

M3 - 文章

AN - SCOPUS:34548106897

SN - 1001-604X

VL - 25

SP - 953

EP - 957

JO - Chinese Journal of Chemistry

JF - Chinese Journal of Chemistry

IS - 7

ER -

Amperometric sensor based on neutral red-doped silica nanoparticles coupled with microdialysis for the measurement of glutamate in the rat striatum

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this