Amperometric sensor based on ferrocene-doped silica nanoparticles as an electron transfer mediator for the determination of glucose in rat brain coupled to in vivo microdialysis

Fen Fen Zhang; Qiao Wan; Xiao Li Wang; Zheng Dong Sun; Zi Qiang Zhu; Yue Zhong Xian; Li Tong Jin; Katsunobu Yamamoto

doi:10.1016/j.jelechem.2004.04.019

Amperometric sensor based on ferrocene-doped silica nanoparticles as an electron transfer mediator for the determination of glucose in rat brain coupled to in vivo microdialysis

Fen Fen Zhang, Qiao Wan, Xiao Li Wang, Zheng Dong Sun, Zi Qiang Zhu, Yue Zhong Xian, Li Tong Jin, Katsunobu Yamamoto

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

In this paper, microdialysis sampling was combined with an enzymatic assay of glucose level in rat brain. A novel amperometric glucose biosensor based on ferrocene-doped silica (FcDS) nanoparticles conjugated with a biopolymer chitosan (CHIT) membrane was developed. These uniform FcDS nanoparticles (about 15±3 nm) were prepared by a water-in-oil (W/O) microemulsion method and were characterized by TEM and electrochemical technology. The nanosilica surface exhibited high biocompatability and the ferrocene doped inside maintained its high electron-transfer efficiency as a mediator. The glucose biosensor showed a detection limit of 2.0×10^-6 molL ^-1 with a linear range from 5.0×10^-6 to 1.2×10^-2 molL^-1. Coupled to microdialysis, it was used to determine the glucose concentration in rat brain. The result was in satisfactory agreement with the literature.

Original language	English
Pages (from-to)	133-138
Number of pages	6
Journal	Journal of Electroanalytical Chemistry
Volume	571
Issue number	2
DOIs	https://doi.org/10.1016/j.jelechem.2004.04.019
State	Published - 1 Oct 2004

Keywords

Electron transfer mediator
Ferrocene-doped silica nanoparticle
Glucose oxidase
Microdialysis sampling
Rat brain

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10.1016/j.jelechem.2004.04.019

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Zhang, F. F., Wan, Q., Wang, X. L., Sun, Z. D., Zhu, Z. Q., Xian, Y. Z., Jin, L. T., & Yamamoto, K. (2004). Amperometric sensor based on ferrocene-doped silica nanoparticles as an electron transfer mediator for the determination of glucose in rat brain coupled to in vivo microdialysis. Journal of Electroanalytical Chemistry, 571(2), 133-138. https://doi.org/10.1016/j.jelechem.2004.04.019

@article{b71243a0a32a409ba482ca2efa8c4bfe,

title = "Amperometric sensor based on ferrocene-doped silica nanoparticles as an electron transfer mediator for the determination of glucose in rat brain coupled to in vivo microdialysis",

abstract = "In this paper, microdialysis sampling was combined with an enzymatic assay of glucose level in rat brain. A novel amperometric glucose biosensor based on ferrocene-doped silica (FcDS) nanoparticles conjugated with a biopolymer chitosan (CHIT) membrane was developed. These uniform FcDS nanoparticles (about 15±3 nm) were prepared by a water-in-oil (W/O) microemulsion method and were characterized by TEM and electrochemical technology. The nanosilica surface exhibited high biocompatability and the ferrocene doped inside maintained its high electron-transfer efficiency as a mediator. The glucose biosensor showed a detection limit of 2.0×10-6 molL -1 with a linear range from 5.0×10-6 to 1.2×10-2 molL-1. Coupled to microdialysis, it was used to determine the glucose concentration in rat brain. The result was in satisfactory agreement with the literature.",

keywords = "Electron transfer mediator, Ferrocene-doped silica nanoparticle, Glucose oxidase, Microdialysis sampling, Rat brain",

author = "Zhang, \{Fen Fen\} and Qiao Wan and Wang, \{Xiao Li\} and Sun, \{Zheng Dong\} and Zhu, \{Zi Qiang\} and Xian, \{Yue Zhong\} and Jin, \{Li Tong\} and Katsunobu Yamamoto",

year = "2004",

month = oct,

day = "1",

doi = "10.1016/j.jelechem.2004.04.019",

language = "英语",

volume = "571",

pages = "133--138",

journal = "Journal of Electroanalytical Chemistry",

issn = "1572-6657",

publisher = "Elsevier B.V.",

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}

Zhang, FF, Wan, Q, Wang, XL, Sun, ZD, Zhu, ZQ, Xian, YZ, Jin, LT & Yamamoto, K 2004, 'Amperometric sensor based on ferrocene-doped silica nanoparticles as an electron transfer mediator for the determination of glucose in rat brain coupled to in vivo microdialysis', Journal of Electroanalytical Chemistry, vol. 571, no. 2, pp. 133-138. https://doi.org/10.1016/j.jelechem.2004.04.019

Amperometric sensor based on ferrocene-doped silica nanoparticles as an electron transfer mediator for the determination of glucose in rat brain coupled to in vivo microdialysis. / Zhang, Fen Fen; Wan, Qiao; Wang, Xiao Li et al.
In: Journal of Electroanalytical Chemistry, Vol. 571, No. 2, 01.10.2004, p. 133-138.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Amperometric sensor based on ferrocene-doped silica nanoparticles as an electron transfer mediator for the determination of glucose in rat brain coupled to in vivo microdialysis

AU - Zhang, Fen Fen

AU - Wan, Qiao

AU - Wang, Xiao Li

AU - Sun, Zheng Dong

AU - Zhu, Zi Qiang

AU - Xian, Yue Zhong

AU - Jin, Li Tong

AU - Yamamoto, Katsunobu

PY - 2004/10/1

Y1 - 2004/10/1

N2 - In this paper, microdialysis sampling was combined with an enzymatic assay of glucose level in rat brain. A novel amperometric glucose biosensor based on ferrocene-doped silica (FcDS) nanoparticles conjugated with a biopolymer chitosan (CHIT) membrane was developed. These uniform FcDS nanoparticles (about 15±3 nm) were prepared by a water-in-oil (W/O) microemulsion method and were characterized by TEM and electrochemical technology. The nanosilica surface exhibited high biocompatability and the ferrocene doped inside maintained its high electron-transfer efficiency as a mediator. The glucose biosensor showed a detection limit of 2.0×10-6 molL -1 with a linear range from 5.0×10-6 to 1.2×10-2 molL-1. Coupled to microdialysis, it was used to determine the glucose concentration in rat brain. The result was in satisfactory agreement with the literature.

AB - In this paper, microdialysis sampling was combined with an enzymatic assay of glucose level in rat brain. A novel amperometric glucose biosensor based on ferrocene-doped silica (FcDS) nanoparticles conjugated with a biopolymer chitosan (CHIT) membrane was developed. These uniform FcDS nanoparticles (about 15±3 nm) were prepared by a water-in-oil (W/O) microemulsion method and were characterized by TEM and electrochemical technology. The nanosilica surface exhibited high biocompatability and the ferrocene doped inside maintained its high electron-transfer efficiency as a mediator. The glucose biosensor showed a detection limit of 2.0×10-6 molL -1 with a linear range from 5.0×10-6 to 1.2×10-2 molL-1. Coupled to microdialysis, it was used to determine the glucose concentration in rat brain. The result was in satisfactory agreement with the literature.

KW - Electron transfer mediator

KW - Ferrocene-doped silica nanoparticle

KW - Glucose oxidase

KW - Microdialysis sampling

KW - Rat brain

UR - https://www.scopus.com/pages/publications/4444258933

U2 - 10.1016/j.jelechem.2004.04.019

DO - 10.1016/j.jelechem.2004.04.019

M3 - 文章

AN - SCOPUS:4444258933

SN - 1572-6657

VL - 571

SP - 133

EP - 138

JO - Journal of Electroanalytical Chemistry

JF - Journal of Electroanalytical Chemistry

IS - 2

ER -

Amperometric sensor based on ferrocene-doped silica nanoparticles as an electron transfer mediator for the determination of glucose in rat brain coupled to in vivo microdialysis

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Keywords

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