TY - JOUR
T1 - Amperometric method for rapid detection of Escherichia coli by flow injection analysis using a bismuth nano-film modified glassy carbon electrode
AU - Zhang, Wen
AU - Tang, Hui
AU - Geng, Ping
AU - Wang, Qingjiang
AU - Jin, Litong
AU - Wu, Zirong
PY - 2007/4
Y1 - 2007/4
N2 - A new method for rapid detection of Escherichia coli (E. coli) was developed by flow injection analysis (FIA) using bismuth nano-film modified glassy carbon electrode (BiNFE) in this paper. The method depended on a good marker β-d-glucuronidase which is found in E. coli strains. β-d-Glucuronidase was produced by the induction of methyl-β-d-glucuronide sodium (MetGlu), then released from E. coli cells through the permeabilization of cell membrane caused by polymyxin B nonapeptide and lysozyme. The released β-d-glucuronidase could catalyze the hydrolysis of the substrate 4-nitrophenyl β-d-glucuronide (PNPG) in the culture medium to produce 4-nitrophenol. Since 4-nitrophenol is electroactive and its quantity is proportional to the concentration of E. coli, E. coli could be determined by electroanalysis of 4-nitrophenol. The BiNFE was fabricated by an electrodeposition of metallic bismuth onto a glassy carbon electrode, which showed a high sensitivity in determination of 4-nitrophenol when used in conjunction with FIA system. Experimental results showed that the amplified response current of 4-nitrophenol obtained at the BiNFE was linear with the concentration of E. coli ranging from 1.5 × 102 to 1.0 × 106 cfu/ml, the detection limit of this method for E. coli is 100 cfu/ml, and the complete assay was performed in 3 h.
AB - A new method for rapid detection of Escherichia coli (E. coli) was developed by flow injection analysis (FIA) using bismuth nano-film modified glassy carbon electrode (BiNFE) in this paper. The method depended on a good marker β-d-glucuronidase which is found in E. coli strains. β-d-Glucuronidase was produced by the induction of methyl-β-d-glucuronide sodium (MetGlu), then released from E. coli cells through the permeabilization of cell membrane caused by polymyxin B nonapeptide and lysozyme. The released β-d-glucuronidase could catalyze the hydrolysis of the substrate 4-nitrophenyl β-d-glucuronide (PNPG) in the culture medium to produce 4-nitrophenol. Since 4-nitrophenol is electroactive and its quantity is proportional to the concentration of E. coli, E. coli could be determined by electroanalysis of 4-nitrophenol. The BiNFE was fabricated by an electrodeposition of metallic bismuth onto a glassy carbon electrode, which showed a high sensitivity in determination of 4-nitrophenol when used in conjunction with FIA system. Experimental results showed that the amplified response current of 4-nitrophenol obtained at the BiNFE was linear with the concentration of E. coli ranging from 1.5 × 102 to 1.0 × 106 cfu/ml, the detection limit of this method for E. coli is 100 cfu/ml, and the complete assay was performed in 3 h.
KW - Amperometric biosensor
KW - Bismuth nano-film modified electrode
KW - Escherichia coli
KW - Flow injection analysis
UR - https://www.scopus.com/pages/publications/33947724920
U2 - 10.1016/j.elecom.2006.11.019
DO - 10.1016/j.elecom.2006.11.019
M3 - 文章
AN - SCOPUS:33947724920
SN - 1388-2481
VL - 9
SP - 833
EP - 838
JO - Electrochemistry Communications
JF - Electrochemistry Communications
IS - 4
ER -