Amantadine-resistance among H5N1 avian influenza viruses isolated in Northern China

Guimei He; Jian Qiao; Changgui Dong; Cheng He; Lihong Zhao; Yong Tian

doi:10.1016/j.antiviral.2007.08.007

Amantadine-resistance among H5N1 avian influenza viruses isolated in Northern China

Guimei He
, Jian Qiao^*
, Changgui Dong
, Cheng He
, Lihong Zhao
, Yong Tian

^*Corresponding author for this work

China Agricultural University

Research output: Contribution to journal › Article › peer-review

142 Scopus citations

Abstract

We tested the amantadine-resistance among avian influenza A (H5N1) viruses isolated from chicken in Hebei Province of Northern China from 2001 to 2005, and investigated the amantadine use in this area. Plague reduction assay in MDCK cells showed that 83.3% isolates (5/6) were amantadine-resistant strains. The M2 sequence analysis revealed that four of five resistant isolates contained the point mutations (Ser to Asn) at position 31 that could confer resistance to amantadine. These results indicated that the incidence of amantadine-resistant viruses isolated in Northern China was particularly high. In the investigation of amantadine use, we found that amantadine was used extensively in poultry farms in this area, which maybe was one of reasons of the high amantadine-resistance incidence.

Original language	English
Pages (from-to)	72-76
Number of pages	5
Journal	Antiviral Research
Volume	77
Issue number	1
DOIs	https://doi.org/10.1016/j.antiviral.2007.08.007
State	Published - Jan 2008
Externally published	Yes

Keywords

Amantadine-resistance
H5N1 avian influenza viruses
M2 gene
Northern China

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10.1016/j.antiviral.2007.08.007

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title = "Amantadine-resistance among H5N1 avian influenza viruses isolated in Northern China",

abstract = "We tested the amantadine-resistance among avian influenza A (H5N1) viruses isolated from chicken in Hebei Province of Northern China from 2001 to 2005, and investigated the amantadine use in this area. Plague reduction assay in MDCK cells showed that 83.3\% isolates (5/6) were amantadine-resistant strains. The M2 sequence analysis revealed that four of five resistant isolates contained the point mutations (Ser to Asn) at position 31 that could confer resistance to amantadine. These results indicated that the incidence of amantadine-resistant viruses isolated in Northern China was particularly high. In the investigation of amantadine use, we found that amantadine was used extensively in poultry farms in this area, which maybe was one of reasons of the high amantadine-resistance incidence.",

keywords = "Amantadine-resistance, H5N1 avian influenza viruses, M2 gene, Northern China",

author = "Guimei He and Jian Qiao and Changgui Dong and Cheng He and Lihong Zhao and Yong Tian",

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doi = "10.1016/j.antiviral.2007.08.007",

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T1 - Amantadine-resistance among H5N1 avian influenza viruses isolated in Northern China

AU - He, Guimei

AU - Qiao, Jian

AU - Dong, Changgui

AU - He, Cheng

AU - Zhao, Lihong

AU - Tian, Yong

PY - 2008/1

Y1 - 2008/1

N2 - We tested the amantadine-resistance among avian influenza A (H5N1) viruses isolated from chicken in Hebei Province of Northern China from 2001 to 2005, and investigated the amantadine use in this area. Plague reduction assay in MDCK cells showed that 83.3% isolates (5/6) were amantadine-resistant strains. The M2 sequence analysis revealed that four of five resistant isolates contained the point mutations (Ser to Asn) at position 31 that could confer resistance to amantadine. These results indicated that the incidence of amantadine-resistant viruses isolated in Northern China was particularly high. In the investigation of amantadine use, we found that amantadine was used extensively in poultry farms in this area, which maybe was one of reasons of the high amantadine-resistance incidence.

AB - We tested the amantadine-resistance among avian influenza A (H5N1) viruses isolated from chicken in Hebei Province of Northern China from 2001 to 2005, and investigated the amantadine use in this area. Plague reduction assay in MDCK cells showed that 83.3% isolates (5/6) were amantadine-resistant strains. The M2 sequence analysis revealed that four of five resistant isolates contained the point mutations (Ser to Asn) at position 31 that could confer resistance to amantadine. These results indicated that the incidence of amantadine-resistant viruses isolated in Northern China was particularly high. In the investigation of amantadine use, we found that amantadine was used extensively in poultry farms in this area, which maybe was one of reasons of the high amantadine-resistance incidence.

KW - Amantadine-resistance

KW - H5N1 avian influenza viruses

KW - M2 gene

KW - Northern China

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DO - 10.1016/j.antiviral.2007.08.007

M3 - 文章

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SP - 72

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JF - Antiviral Research

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Amantadine-resistance among H5N1 avian influenza viruses isolated in Northern China

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Keywords

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