ADAMTS-18 in the host tissues exerts little effect on breast tumor progress in a murine 4T1 breast cancer model

Min Liu; Tiantian Lu; Fang Jing; Suying Dang; Wei Zhang

doi:10.1186/s12952-016-0045-0

ADAMTS-18 in the host tissues exerts little effect on breast tumor progress in a murine 4T1 breast cancer model

Min Liu
, Tiantian Lu
, Fang Jing
, Suying Dang
, Wei Zhang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Background: In this study, we aimed to identify a novel extracellular proteinase ADAMTS-18 that could be a potential tumor suppressor gene. Results: We successfully constructed Adamts-18 knockout mice with BALB / c background. RT-PCR analysis showed syngeneic mammary tumor cell line 4 T1 per se has weakly endogenous ADAMTS-18 expression. Orthotopic inoculation of 4 T1 cells within the mammary fat pad of host mice, we found no significant difference in tumor growth and metastasis between Adamts-18 knockout mice and widetype control. Conclusions: We did not confirm that ADAMTS-18 in the host tissues is relevant for breast tumor progress in a murine 4 T1 breast cancer model.

Original language	English
Article number	2
Journal	Journal of Negative Results in BioMedicine
Volume	15
Issue number	1
DOIs	https://doi.org/10.1186/s12952-016-0045-0
State	Published - 3 Feb 2016
Externally published	Yes

Keywords

ADAMTS-18
Breast cancer
Knockout mice

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1186/s12952-016-0045-0

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title = "ADAMTS-18 in the host tissues exerts little effect on breast tumor progress in a murine 4T1 breast cancer model",

abstract = "Background: In this study, we aimed to identify a novel extracellular proteinase ADAMTS-18 that could be a potential tumor suppressor gene. Results: We successfully constructed Adamts-18 knockout mice with BALB / c background. RT-PCR analysis showed syngeneic mammary tumor cell line 4 T1 per se has weakly endogenous ADAMTS-18 expression. Orthotopic inoculation of 4 T1 cells within the mammary fat pad of host mice, we found no significant difference in tumor growth and metastasis between Adamts-18 knockout mice and widetype control. Conclusions: We did not confirm that ADAMTS-18 in the host tissues is relevant for breast tumor progress in a murine 4 T1 breast cancer model.",

keywords = "ADAMTS-18, Breast cancer, Knockout mice",

author = "Min Liu and Tiantian Lu and Fang Jing and Suying Dang and Wei Zhang",

note = "Publisher Copyright: {\textcopyright} 2016 Liu et al.",

year = "2016",

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doi = "10.1186/s12952-016-0045-0",

language = "英语",

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TY - JOUR

T1 - ADAMTS-18 in the host tissues exerts little effect on breast tumor progress in a murine 4T1 breast cancer model

AU - Liu, Min

AU - Lu, Tiantian

AU - Jing, Fang

AU - Dang, Suying

AU - Zhang, Wei

PY - 2016/2/3

Y1 - 2016/2/3

N2 - Background: In this study, we aimed to identify a novel extracellular proteinase ADAMTS-18 that could be a potential tumor suppressor gene. Results: We successfully constructed Adamts-18 knockout mice with BALB / c background. RT-PCR analysis showed syngeneic mammary tumor cell line 4 T1 per se has weakly endogenous ADAMTS-18 expression. Orthotopic inoculation of 4 T1 cells within the mammary fat pad of host mice, we found no significant difference in tumor growth and metastasis between Adamts-18 knockout mice and widetype control. Conclusions: We did not confirm that ADAMTS-18 in the host tissues is relevant for breast tumor progress in a murine 4 T1 breast cancer model.

AB - Background: In this study, we aimed to identify a novel extracellular proteinase ADAMTS-18 that could be a potential tumor suppressor gene. Results: We successfully constructed Adamts-18 knockout mice with BALB / c background. RT-PCR analysis showed syngeneic mammary tumor cell line 4 T1 per se has weakly endogenous ADAMTS-18 expression. Orthotopic inoculation of 4 T1 cells within the mammary fat pad of host mice, we found no significant difference in tumor growth and metastasis between Adamts-18 knockout mice and widetype control. Conclusions: We did not confirm that ADAMTS-18 in the host tissues is relevant for breast tumor progress in a murine 4 T1 breast cancer model.

KW - ADAMTS-18

KW - Breast cancer

KW - Knockout mice

UR - https://www.scopus.com/pages/publications/84957958028

U2 - 10.1186/s12952-016-0045-0

DO - 10.1186/s12952-016-0045-0

M3 - 文章

C2 - 26841794

AN - SCOPUS:84957958028

SN - 1477-5751

VL - 15

JO - Journal of Negative Results in BioMedicine

JF - Journal of Negative Results in BioMedicine

IS - 1

M1 - 2

ER -

ADAMTS-18 in the host tissues exerts little effect on breast tumor progress in a murine 4T1 breast cancer model

Abstract

Keywords

UN SDGs

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