Accelerated stochastic processes of plankton community assembly due to tidal restriction by seawall construction in the Yangtze River Estuary

Seawall construction has complex ecological impacts. However, the ecological mechanisms within plankton communities under tidal restriction resulting from seawall construction remain unexplored. Using environmental DNA (eDNA) metabarcoding, this study examined the impact of seawall construction on the assembly process of planktonic eukaryote and bacteria communities from the unrestricted area and the tide-restricted area in the Chongming Dongtan Nature Reserve of Yangtze River Estuary. While environmental heterogeneity did not exert a significant influence on alpha diversity of plankton, it had a significant impact on community structure. Variation partitioning analysis (VPA) and neutral community model indicated that neither environmental nor spatial factors were predominant drivers of plankton community composition and structure, instead, they were influenced by stochastic processes. Moreover, it was observed that the relative significance of stochastic processes in the tide-restricted area exceeded that in the unrestricted area. High habitat uniformity and water connectivity resulting from seawall construction may facilitate homogenization and spread among high-abundance groups. The results have significant implications for understanding the mechanisms underlying succession and composition, and for improving ecological assessment and remediation efforts in areas impacted by tidal restriction.