A two-photon ratiometric fluorescence probe for cupric ions in live cells and tissues

Anwei Zhu; Changqin Ding; Yang Tian

doi:10.1038/srep02933

A two-photon ratiometric fluorescence probe for cupric ions in live cells and tissues

Anwei Zhu
, Changqin Ding
, Yang Tian^*

^*Corresponding author for this work

Tongji University

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Development of sensitive and selective probes for cupric ions (Cu ²⁺) at cell and tissue level is a challenging work for progress in understanding the biological effects of Cu²⁺. Here, we report a ratiometric two-photon probe for Cu²⁺ based on the organic-inorganic hybrids of graphene quantum dots (GQDs) and Nile Blue dye. Meanwhile, Cu-free derivative of copper-zinc superoxide dismutase (SOD)-E 2 Zn 2 SOD is designed as the unique receptor for Cu²⁺ and conjugated on the surface of GQDs. This probe shows a blue-to-yellow color change in repose to Cu²⁺, good selectivity, low cytotoxicity, long-term photostability, and insensitivity to pH over the biologically relevant pH range. The developed probe allows the direct visualization of Cu²⁺ levels in live cells as well as in deep-tissues at 90-180μm depth through the use of two-photon microscopy. Furthermore, the effect of ascorbic acid is also evaluated on intracellular Cu²⁺ binding to E₂ Zn₂ SOD by this probe.

Original language	English
Article number	2933
Journal	Scientific Reports
Volume	3
DOIs	https://doi.org/10.1038/srep02933
State	Published - 2013
Externally published	Yes

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title = "A two-photon ratiometric fluorescence probe for cupric ions in live cells and tissues",

abstract = "Development of sensitive and selective probes for cupric ions (Cu 2+) at cell and tissue level is a challenging work for progress in understanding the biological effects of Cu2+. Here, we report a ratiometric two-photon probe for Cu2+ based on the organic-inorganic hybrids of graphene quantum dots (GQDs) and Nile Blue dye. Meanwhile, Cu-free derivative of copper-zinc superoxide dismutase (SOD)-E 2 Zn 2 SOD is designed as the unique receptor for Cu2+ and conjugated on the surface of GQDs. This probe shows a blue-to-yellow color change in repose to Cu2+, good selectivity, low cytotoxicity, long-term photostability, and insensitivity to pH over the biologically relevant pH range. The developed probe allows the direct visualization of Cu2+ levels in live cells as well as in deep-tissues at 90-180μm depth through the use of two-photon microscopy. Furthermore, the effect of ascorbic acid is also evaluated on intracellular Cu2+ binding to E2 Zn2 SOD by this probe.",

author = "Anwei Zhu and Changqin Ding and Yang Tian",

year = "2013",

doi = "10.1038/srep02933",

language = "英语",

volume = "3",

journal = "Scientific Reports",

issn = "2045-2322",

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T1 - A two-photon ratiometric fluorescence probe for cupric ions in live cells and tissues

AU - Zhu, Anwei

AU - Ding, Changqin

AU - Tian, Yang

PY - 2013

Y1 - 2013

N2 - Development of sensitive and selective probes for cupric ions (Cu 2+) at cell and tissue level is a challenging work for progress in understanding the biological effects of Cu2+. Here, we report a ratiometric two-photon probe for Cu2+ based on the organic-inorganic hybrids of graphene quantum dots (GQDs) and Nile Blue dye. Meanwhile, Cu-free derivative of copper-zinc superoxide dismutase (SOD)-E 2 Zn 2 SOD is designed as the unique receptor for Cu2+ and conjugated on the surface of GQDs. This probe shows a blue-to-yellow color change in repose to Cu2+, good selectivity, low cytotoxicity, long-term photostability, and insensitivity to pH over the biologically relevant pH range. The developed probe allows the direct visualization of Cu2+ levels in live cells as well as in deep-tissues at 90-180μm depth through the use of two-photon microscopy. Furthermore, the effect of ascorbic acid is also evaluated on intracellular Cu2+ binding to E2 Zn2 SOD by this probe.

AB - Development of sensitive and selective probes for cupric ions (Cu 2+) at cell and tissue level is a challenging work for progress in understanding the biological effects of Cu2+. Here, we report a ratiometric two-photon probe for Cu2+ based on the organic-inorganic hybrids of graphene quantum dots (GQDs) and Nile Blue dye. Meanwhile, Cu-free derivative of copper-zinc superoxide dismutase (SOD)-E 2 Zn 2 SOD is designed as the unique receptor for Cu2+ and conjugated on the surface of GQDs. This probe shows a blue-to-yellow color change in repose to Cu2+, good selectivity, low cytotoxicity, long-term photostability, and insensitivity to pH over the biologically relevant pH range. The developed probe allows the direct visualization of Cu2+ levels in live cells as well as in deep-tissues at 90-180μm depth through the use of two-photon microscopy. Furthermore, the effect of ascorbic acid is also evaluated on intracellular Cu2+ binding to E2 Zn2 SOD by this probe.

UR - https://www.scopus.com/pages/publications/84886934011

U2 - 10.1038/srep02933

DO - 10.1038/srep02933

M3 - 文章

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AN - SCOPUS:84886934011

SN - 2045-2322

VL - 3

JO - Scientific Reports

JF - Scientific Reports

M1 - 2933

ER -

A two-photon ratiometric fluorescence probe for cupric ions in live cells and tissues

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