A three-channel fluorescent probe that distinguishes peroxynitrite from hypochlorite

Quanjuan Zhang; Zhichuan Zhu; Yongli Zheng; Jiagao Cheng; Na Zhang; Yi Tao Long; Jing Zheng; Xuhong Qian; Youjun Yang

doi:10.1021/ja305046u

A three-channel fluorescent probe that distinguishes peroxynitrite from hypochlorite

Quanjuan Zhang
, Zhichuan Zhu
, Yongli Zheng
, Jiagao Cheng
, Na Zhang
, Yi Tao Long
, Jing Zheng^*
, Xuhong Qian
, Youjun Yang

^*Corresponding author for this work

East China University of Science and Technology
Shanghai Jiao Tong University

Research output: Contribution to journal › Article › peer-review

172 Scopus citations

Abstract

A novel fluorescent probe for peroxynitrite, PN₆₀₀, was rationally designed on the basis of a unique fluorophore assembly approach. PN₆₀₀ is a green-emitting coumarin derivative. Upon oxidation by peroxynitrite, PN₆₀₀ is transformed into a highly fluorescent red-emitting resorufin derivative via an orange-emitting intermediate. This three-channel signaling capability enables PN₆₀₀ to differentiate peroxynitrite from other reactive oxygen and nitrogen species, including hypochlorite and hydroxyl radical. Moreover, PN₆₀₀ is membrane-permeable and compatible with common TRITC filter sets and displays low cytotoxicity. Therefore, PN₆₀₀ is a promising candidate for in vitro peroxynitrite imaging.

Original language	English
Pages (from-to)	18479-18482
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	134
Issue number	45
DOIs	https://doi.org/10.1021/ja305046u
State	Published - 14 Nov 2012
Externally published	Yes

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title = "A three-channel fluorescent probe that distinguishes peroxynitrite from hypochlorite",

abstract = "A novel fluorescent probe for peroxynitrite, PN600, was rationally designed on the basis of a unique fluorophore assembly approach. PN600 is a green-emitting coumarin derivative. Upon oxidation by peroxynitrite, PN600 is transformed into a highly fluorescent red-emitting resorufin derivative via an orange-emitting intermediate. This three-channel signaling capability enables PN600 to differentiate peroxynitrite from other reactive oxygen and nitrogen species, including hypochlorite and hydroxyl radical. Moreover, PN600 is membrane-permeable and compatible with common TRITC filter sets and displays low cytotoxicity. Therefore, PN600 is a promising candidate for in vitro peroxynitrite imaging.",

author = "Quanjuan Zhang and Zhichuan Zhu and Yongli Zheng and Jiagao Cheng and Na Zhang and Long, \{Yi Tao\} and Jing Zheng and Xuhong Qian and Youjun Yang",

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T1 - A three-channel fluorescent probe that distinguishes peroxynitrite from hypochlorite

AU - Zhang, Quanjuan

AU - Zhu, Zhichuan

AU - Zheng, Yongli

AU - Cheng, Jiagao

AU - Zhang, Na

AU - Long, Yi Tao

AU - Zheng, Jing

AU - Qian, Xuhong

AU - Yang, Youjun

PY - 2012/11/14

Y1 - 2012/11/14

N2 - A novel fluorescent probe for peroxynitrite, PN600, was rationally designed on the basis of a unique fluorophore assembly approach. PN600 is a green-emitting coumarin derivative. Upon oxidation by peroxynitrite, PN600 is transformed into a highly fluorescent red-emitting resorufin derivative via an orange-emitting intermediate. This three-channel signaling capability enables PN600 to differentiate peroxynitrite from other reactive oxygen and nitrogen species, including hypochlorite and hydroxyl radical. Moreover, PN600 is membrane-permeable and compatible with common TRITC filter sets and displays low cytotoxicity. Therefore, PN600 is a promising candidate for in vitro peroxynitrite imaging.

AB - A novel fluorescent probe for peroxynitrite, PN600, was rationally designed on the basis of a unique fluorophore assembly approach. PN600 is a green-emitting coumarin derivative. Upon oxidation by peroxynitrite, PN600 is transformed into a highly fluorescent red-emitting resorufin derivative via an orange-emitting intermediate. This three-channel signaling capability enables PN600 to differentiate peroxynitrite from other reactive oxygen and nitrogen species, including hypochlorite and hydroxyl radical. Moreover, PN600 is membrane-permeable and compatible with common TRITC filter sets and displays low cytotoxicity. Therefore, PN600 is a promising candidate for in vitro peroxynitrite imaging.

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DO - 10.1021/ja305046u

M3 - 文章

C2 - 22978649

AN - SCOPUS:84869458969

SN - 0002-7863

VL - 134

SP - 18479

EP - 18482

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 45

ER -

A three-channel fluorescent probe that distinguishes peroxynitrite from hypochlorite

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