TY - JOUR
T1 - A smart glycol-directed nanodevice from rationally designed macroporous materials
AU - Qian, Kun
AU - Wan, Jingjing
AU - Huang, Xiaodan
AU - Yang, Pengyuan
AU - Liu, Baohong
AU - Yu, Chengzhong
PY - 2010/1/18
Y1 - 2010/1/18
N2 - We have developed a smart nanodevice for the highly efficient and selective detection of glycoproteins. This polyfunctional device is fabricated through the rational functionalization of macroporous silica foam (MOSF) materials with a boron species (B- MOSF) and amino groups (NH2- MOSF), and then the integration of MOSF, B-MOSF and NH2-MOSF materials. In such a device, a macroporous structure with very large-pore sizes (di- ameters≈100 nm) and high-pore volumes (> 0.65 cm3 g-1) is advantageous to efficiently fasten the enzymatic reaction. The targeted specific glycopepti- des of the products can be selectively isolated and enriched in B-MOSF through the chemo-affinity between boronic acid and glycol groups, while the non-specific peptides are released to the solutions, or further purified by MOSF and NH 2-MOSF, which have opposite charges. As a result, the protein digestion and glycol-peptide isolation can be simultaneously achieved in the functionalized macroporous materials in one step, which is a great advantage compared to conventional multi-procedure and time-consuming techniques.
AB - We have developed a smart nanodevice for the highly efficient and selective detection of glycoproteins. This polyfunctional device is fabricated through the rational functionalization of macroporous silica foam (MOSF) materials with a boron species (B- MOSF) and amino groups (NH2- MOSF), and then the integration of MOSF, B-MOSF and NH2-MOSF materials. In such a device, a macroporous structure with very large-pore sizes (di- ameters≈100 nm) and high-pore volumes (> 0.65 cm3 g-1) is advantageous to efficiently fasten the enzymatic reaction. The targeted specific glycopepti- des of the products can be selectively isolated and enriched in B-MOSF through the chemo-affinity between boronic acid and glycol groups, while the non-specific peptides are released to the solutions, or further purified by MOSF and NH 2-MOSF, which have opposite charges. As a result, the protein digestion and glycol-peptide isolation can be simultaneously achieved in the functionalized macroporous materials in one step, which is a great advantage compared to conventional multi-procedure and time-consuming techniques.
KW - Gglycosylation
KW - Macroporous materials
KW - Nanotechnology
UR - https://www.scopus.com/pages/publications/75249107292
U2 - 10.1002/chem.200902535
DO - 10.1002/chem.200902535
M3 - 文章
C2 - 20024995
AN - SCOPUS:75249107292
SN - 0947-6539
VL - 16
SP - 822
EP - 828
JO - Chemistry - A European Journal
JF - Chemistry - A European Journal
IS - 3
ER -