A ratiometric fluorescent probe for alkaline phosphatase with high sensitivity

Yang Yangyang; Zhang Chen; Pan Rizhao; Zhang Shiwei; Yao Shengtao; Tang Yao; Zhu Weilong; Wang Liyue; Zhu Weiping; Xu Yufang; Qian Xuhong

doi:10.1016/j.cclet.2019.04.037

A ratiometric fluorescent probe for alkaline phosphatase with high sensitivity

Yang Yangyang^*
, Zhang Chen
, Pan Rizhao
, Zhang Shiwei
, Yao Shengtao
, Tang Yao
, Zhu Weilong
, Wang Liyue
, Zhu Weiping
, Xu Yufang
, Qian Xuhong^*

^*Corresponding author for this work

East China University of Science and Technology

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Alkaline phosphatase (ALP) is one of essential biomarkers in mammalian tissue. Here we report a ratiometric probe for ALP, which is rationally designed and synthesized by employing ESIPT fluorophore N-(3-(benzo[d]thiazol-2-yl)-4-hydroxyphenyl)benzamide (BTHPB). The enzymatic dephosphorylation converts the probe to BTHPB, which exhibits a large spectral red-shift (120 nm), allowing extremely high sensitivity of ALP sensing at 0.004 mU/mL. The probe also shows excellent biocompatibility and has been applied for monitoring the endogenic ALP in living cells.

Original language	English
Pages (from-to)	125-128
Number of pages	4
Journal	Chinese Chemical Letters
Volume	31
Issue number	1
DOIs	https://doi.org/10.1016/j.cclet.2019.04.037
State	Published - Jan 2020
Externally published	Yes

Keywords

Alkaline phosphatase
Biomarker
ESIPT
Fluorescent probe
Ratiometric

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10.1016/j.cclet.2019.04.037

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title = "A ratiometric fluorescent probe for alkaline phosphatase with high sensitivity",

abstract = "Alkaline phosphatase (ALP) is one of essential biomarkers in mammalian tissue. Here we report a ratiometric probe for ALP, which is rationally designed and synthesized by employing ESIPT fluorophore N-(3-(benzo[d]thiazol-2-yl)-4-hydroxyphenyl)benzamide (BTHPB). The enzymatic dephosphorylation converts the probe to BTHPB, which exhibits a large spectral red-shift (120 nm), allowing extremely high sensitivity of ALP sensing at 0.004 mU/mL. The probe also shows excellent biocompatibility and has been applied for monitoring the endogenic ALP in living cells.",

keywords = "Alkaline phosphatase, Biomarker, ESIPT, Fluorescent probe, Ratiometric",

author = "Yang Yangyang and Zhang Chen and Pan Rizhao and Zhang Shiwei and Yao Shengtao and Tang Yao and Zhu Weilong and Wang Liyue and Zhu Weiping and Xu Yufang and Qian Xuhong",

note = "Publisher Copyright: {\textcopyright} 2019 The Author",

year = "2020",

month = jan,

doi = "10.1016/j.cclet.2019.04.037",

language = "英语",

volume = "31",

pages = "125--128",

journal = "Chinese Chemical Letters",

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TY - JOUR

T1 - A ratiometric fluorescent probe for alkaline phosphatase with high sensitivity

AU - Yangyang, Yang

AU - Chen, Zhang

AU - Rizhao, Pan

AU - Shiwei, Zhang

AU - Shengtao, Yao

AU - Yao, Tang

AU - Weilong, Zhu

AU - Liyue, Wang

AU - Weiping, Zhu

AU - Yufang, Xu

AU - Xuhong, Qian

PY - 2020/1

Y1 - 2020/1

N2 - Alkaline phosphatase (ALP) is one of essential biomarkers in mammalian tissue. Here we report a ratiometric probe for ALP, which is rationally designed and synthesized by employing ESIPT fluorophore N-(3-(benzo[d]thiazol-2-yl)-4-hydroxyphenyl)benzamide (BTHPB). The enzymatic dephosphorylation converts the probe to BTHPB, which exhibits a large spectral red-shift (120 nm), allowing extremely high sensitivity of ALP sensing at 0.004 mU/mL. The probe also shows excellent biocompatibility and has been applied for monitoring the endogenic ALP in living cells.

AB - Alkaline phosphatase (ALP) is one of essential biomarkers in mammalian tissue. Here we report a ratiometric probe for ALP, which is rationally designed and synthesized by employing ESIPT fluorophore N-(3-(benzo[d]thiazol-2-yl)-4-hydroxyphenyl)benzamide (BTHPB). The enzymatic dephosphorylation converts the probe to BTHPB, which exhibits a large spectral red-shift (120 nm), allowing extremely high sensitivity of ALP sensing at 0.004 mU/mL. The probe also shows excellent biocompatibility and has been applied for monitoring the endogenic ALP in living cells.

KW - Alkaline phosphatase

KW - Biomarker

KW - ESIPT

KW - Fluorescent probe

KW - Ratiometric

UR - https://www.scopus.com/pages/publications/85065017226

U2 - 10.1016/j.cclet.2019.04.037

DO - 10.1016/j.cclet.2019.04.037

M3 - 文章

AN - SCOPUS:85065017226

SN - 1001-8417

VL - 31

SP - 125

EP - 128

JO - Chinese Chemical Letters

JF - Chinese Chemical Letters

IS - 1

ER -

A ratiometric fluorescent probe for alkaline phosphatase with high sensitivity

Abstract

Keywords

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