A ratiometric fluorescent probe based on FRET for imaging Hg2+ ions in living cells

Xiaolin Zhang; Yi Xiao; Xuhong Qian

doi:10.1002/anie.200803246

A ratiometric fluorescent probe based on FRET for imaging Hg²⁺ ions in living cells

Xiaolin Zhang^*
, Yi Xiao
, Xuhong Qian

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

814 Scopus citations

Abstract

(Chemical Equation Presented) Mercury rising: A FRET-based ratiometric probe (see picture; FRET = fluorescence resonance energy transfer) can selectively detect Hg²⁺ ions on the parts-per-billion scale under physiological conditions. The probe undergoes a clear Hg²⁺-induced change in the intensity ratio ofthe two well-separated and equally intense emission bands of dipyrrometheneboron difluoride and rhodamine. Experiments show that the probe can be used in living cells.

Original language	English
Pages (from-to)	8025-8029
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	47
Issue number	42
DOIs	https://doi.org/10.1002/anie.200803246
State	Published - 6 Oct 2008
Externally published	Yes

Keywords

FRET
Fluorescent probes
Mercury
Rhodamine
Sensors

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10.1002/anie.200803246

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abstract = "(Chemical Equation Presented) Mercury rising: A FRET-based ratiometric probe (see picture; FRET = fluorescence resonance energy transfer) can selectively detect Hg2+ ions on the parts-per-billion scale under physiological conditions. The probe undergoes a clear Hg2+-induced change in the intensity ratio ofthe two well-separated and equally intense emission bands of dipyrrometheneboron difluoride and rhodamine. Experiments show that the probe can be used in living cells.",

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author = "Xiaolin Zhang and Yi Xiao and Xuhong Qian",

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AU - Zhang, Xiaolin

AU - Xiao, Yi

AU - Qian, Xuhong

PY - 2008/10/6

Y1 - 2008/10/6

N2 - (Chemical Equation Presented) Mercury rising: A FRET-based ratiometric probe (see picture; FRET = fluorescence resonance energy transfer) can selectively detect Hg2+ ions on the parts-per-billion scale under physiological conditions. The probe undergoes a clear Hg2+-induced change in the intensity ratio ofthe two well-separated and equally intense emission bands of dipyrrometheneboron difluoride and rhodamine. Experiments show that the probe can be used in living cells.

AB - (Chemical Equation Presented) Mercury rising: A FRET-based ratiometric probe (see picture; FRET = fluorescence resonance energy transfer) can selectively detect Hg2+ ions on the parts-per-billion scale under physiological conditions. The probe undergoes a clear Hg2+-induced change in the intensity ratio ofthe two well-separated and equally intense emission bands of dipyrrometheneboron difluoride and rhodamine. Experiments show that the probe can be used in living cells.

KW - FRET

KW - Fluorescent probes

KW - Mercury

KW - Rhodamine

KW - Sensors

UR - https://www.scopus.com/pages/publications/54049138881

U2 - 10.1002/anie.200803246

DO - 10.1002/anie.200803246

M3 - 文章

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AN - SCOPUS:54049138881

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JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 42

ER -

A ratiometric fluorescent probe based on FRET for imaging Hg²⁺ ions in living cells

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Keywords

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