A polyclonal antibody against active c-terminal adamts-18 fragment

Suying Dang; Dawei Bu; Tao Hong; Wei Zhang

doi:10.1089/hyb.2011.0055

A polyclonal antibody against active c-terminal adamts-18 fragment

Suying Dang
, Dawei Bu
, Tao Hong
, Wei Zhang^*

^*Corresponding author for this work

School of Life Sciences

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The human ADAMTS-18, a disintegrin and metalloproteinase with thrombospondin type-1 modules 18, is a secreted Zn-metalloproteinase. The C-terminal 385-amino acid fragment of ADAMTS-18 (AD18C) is highly effective at promoting platelet thrombus dissolution in vivo. Therefore, polyclonal antibody (pAb) against AD18C fragment should be able to keep platelet thrombus stability, which has direct clinical relevance. In this report, pAb against AD18C fragment was generated from rabbit immunized with AD18C recombinant protein (rAD18C). The pAb showed specific binding with rAD18C and natural ADAMTS-18 protein by ELISA and Western blot assay. It shortens the mouse tail bleeding time in a dose-dependent manner. Thus, anti-AD18C pAb contributes to the regulation of platelet thrombus stability.

Original language	English
Pages (from-to)	567-569
Number of pages	3
Journal	Hybridoma
Volume	30
Issue number	6
DOIs	https://doi.org/10.1089/hyb.2011.0055
State	Published - 1 Dec 2011

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title = "A polyclonal antibody against active c-terminal adamts-18 fragment",

abstract = "The human ADAMTS-18, a disintegrin and metalloproteinase with thrombospondin type-1 modules 18, is a secreted Zn-metalloproteinase. The C-terminal 385-amino acid fragment of ADAMTS-18 (AD18C) is highly effective at promoting platelet thrombus dissolution in vivo. Therefore, polyclonal antibody (pAb) against AD18C fragment should be able to keep platelet thrombus stability, which has direct clinical relevance. In this report, pAb against AD18C fragment was generated from rabbit immunized with AD18C recombinant protein (rAD18C). The pAb showed specific binding with rAD18C and natural ADAMTS-18 protein by ELISA and Western blot assay. It shortens the mouse tail bleeding time in a dose-dependent manner. Thus, anti-AD18C pAb contributes to the regulation of platelet thrombus stability.",

author = "Suying Dang and Dawei Bu and Tao Hong and Wei Zhang",

year = "2011",

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doi = "10.1089/hyb.2011.0055",

language = "英语",

volume = "30",

pages = "567--569",

journal = "Hybridoma",

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TY - JOUR

T1 - A polyclonal antibody against active c-terminal adamts-18 fragment

AU - Dang, Suying

AU - Bu, Dawei

AU - Hong, Tao

AU - Zhang, Wei

PY - 2011/12/1

Y1 - 2011/12/1

N2 - The human ADAMTS-18, a disintegrin and metalloproteinase with thrombospondin type-1 modules 18, is a secreted Zn-metalloproteinase. The C-terminal 385-amino acid fragment of ADAMTS-18 (AD18C) is highly effective at promoting platelet thrombus dissolution in vivo. Therefore, polyclonal antibody (pAb) against AD18C fragment should be able to keep platelet thrombus stability, which has direct clinical relevance. In this report, pAb against AD18C fragment was generated from rabbit immunized with AD18C recombinant protein (rAD18C). The pAb showed specific binding with rAD18C and natural ADAMTS-18 protein by ELISA and Western blot assay. It shortens the mouse tail bleeding time in a dose-dependent manner. Thus, anti-AD18C pAb contributes to the regulation of platelet thrombus stability.

AB - The human ADAMTS-18, a disintegrin and metalloproteinase with thrombospondin type-1 modules 18, is a secreted Zn-metalloproteinase. The C-terminal 385-amino acid fragment of ADAMTS-18 (AD18C) is highly effective at promoting platelet thrombus dissolution in vivo. Therefore, polyclonal antibody (pAb) against AD18C fragment should be able to keep platelet thrombus stability, which has direct clinical relevance. In this report, pAb against AD18C fragment was generated from rabbit immunized with AD18C recombinant protein (rAD18C). The pAb showed specific binding with rAD18C and natural ADAMTS-18 protein by ELISA and Western blot assay. It shortens the mouse tail bleeding time in a dose-dependent manner. Thus, anti-AD18C pAb contributes to the regulation of platelet thrombus stability.

UR - https://www.scopus.com/pages/publications/83455186408

U2 - 10.1089/hyb.2011.0055

DO - 10.1089/hyb.2011.0055

M3 - 文章

C2 - 22149285

AN - SCOPUS:83455186408

SN - 1554-0014

VL - 30

SP - 567

EP - 569

JO - Hybridoma

JF - Hybridoma

IS - 6

ER -

A polyclonal antibody against active c-terminal adamts-18 fragment

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