A phenylboronate-based SERS nanoprobe for detection and imaging of intracellular peroxynitrite

Hua Ying Chen; Dan Guo; Zhen Fei Gan; Lei Jiang; Shuai Chang; Da Wei Li

doi:10.1007/s00604-018-3129-3

A phenylboronate-based SERS nanoprobe for detection and imaging of intracellular peroxynitrite

Hua Ying Chen
, Dan Guo
, Zhen Fei Gan
, Lei Jiang
, Shuai Chang
, Da Wei Li^*

^*Corresponding author for this work

East China University of Science and Technology

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

A surface-enhanced Raman scattering (SERS) based nanoprobe was developed for detection and imaging of endogenous peroxynitrite in living cells. The probe was fabricated by assembling 3-mercaptophenylboronic acid pinacol ester onto the surface of gold nanoparticles (AuNPs). The detection of peroxynitrite is accomplished via measurement of the changes in the SERS spectra (at 882 cm⁻¹) that are caused by the reaction between probe and peroxynitrite. The probe has a fast response (<30 s), a 0.4 μM lower detection limit and a wide linearity range from 5.0 × 10⁻⁷ to 1.0 × 10⁻⁴ M. It is biocompatible and highly stable on storage and under various pH conditions. Both the reaction and the SERS signal are highly specific over other species. The nanoprobe was successfully applied to SERS imaging of peroxynitrite that is produced in macrophages under oxidative stress. Conceivably, the method has a most viable tool for use in studies on peroxynitrite-related physiological and pathological processes. [Figure not available: see fulltext.].

Original language	English
Article number	11
Journal	Microchimica Acta
Volume	186
Issue number	1
DOIs	https://doi.org/10.1007/s00604-018-3129-3
State	Published - 1 Jan 2019
Externally published	Yes

Keywords

Biosensor
Boronate ester
Gold nanoparticles
Living cell
Oxidative stress
Reactive oxygen species
Specific reaction
Surface-enhanced Raman scattering

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10.1007/s00604-018-3129-3

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title = "A phenylboronate-based SERS nanoprobe for detection and imaging of intracellular peroxynitrite",

abstract = "A surface-enhanced Raman scattering (SERS) based nanoprobe was developed for detection and imaging of endogenous peroxynitrite in living cells. The probe was fabricated by assembling 3-mercaptophenylboronic acid pinacol ester onto the surface of gold nanoparticles (AuNPs). The detection of peroxynitrite is accomplished via measurement of the changes in the SERS spectra (at 882 cm−1) that are caused by the reaction between probe and peroxynitrite. The probe has a fast response (<30 s), a 0.4 μM lower detection limit and a wide linearity range from 5.0 × 10−7 to 1.0 × 10−4 M. It is biocompatible and highly stable on storage and under various pH conditions. Both the reaction and the SERS signal are highly specific over other species. The nanoprobe was successfully applied to SERS imaging of peroxynitrite that is produced in macrophages under oxidative stress. Conceivably, the method has a most viable tool for use in studies on peroxynitrite-related physiological and pathological processes. [Figure not available: see fulltext.].",

keywords = "Biosensor, Boronate ester, Gold nanoparticles, Living cell, Oxidative stress, Reactive oxygen species, Specific reaction, Surface-enhanced Raman scattering",

author = "Chen, \{Hua Ying\} and Dan Guo and Gan, \{Zhen Fei\} and Lei Jiang and Shuai Chang and Li, \{Da Wei\}",

note = "Publisher Copyright: {\textcopyright} 2018, Springer-Verlag GmbH Austria, part of Springer Nature.",

year = "2019",

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doi = "10.1007/s00604-018-3129-3",

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T1 - A phenylboronate-based SERS nanoprobe for detection and imaging of intracellular peroxynitrite

AU - Chen, Hua Ying

AU - Guo, Dan

AU - Gan, Zhen Fei

AU - Jiang, Lei

AU - Chang, Shuai

AU - Li, Da Wei

PY - 2019/1/1

Y1 - 2019/1/1

N2 - A surface-enhanced Raman scattering (SERS) based nanoprobe was developed for detection and imaging of endogenous peroxynitrite in living cells. The probe was fabricated by assembling 3-mercaptophenylboronic acid pinacol ester onto the surface of gold nanoparticles (AuNPs). The detection of peroxynitrite is accomplished via measurement of the changes in the SERS spectra (at 882 cm−1) that are caused by the reaction between probe and peroxynitrite. The probe has a fast response (<30 s), a 0.4 μM lower detection limit and a wide linearity range from 5.0 × 10−7 to 1.0 × 10−4 M. It is biocompatible and highly stable on storage and under various pH conditions. Both the reaction and the SERS signal are highly specific over other species. The nanoprobe was successfully applied to SERS imaging of peroxynitrite that is produced in macrophages under oxidative stress. Conceivably, the method has a most viable tool for use in studies on peroxynitrite-related physiological and pathological processes. [Figure not available: see fulltext.].

AB - A surface-enhanced Raman scattering (SERS) based nanoprobe was developed for detection and imaging of endogenous peroxynitrite in living cells. The probe was fabricated by assembling 3-mercaptophenylboronic acid pinacol ester onto the surface of gold nanoparticles (AuNPs). The detection of peroxynitrite is accomplished via measurement of the changes in the SERS spectra (at 882 cm−1) that are caused by the reaction between probe and peroxynitrite. The probe has a fast response (<30 s), a 0.4 μM lower detection limit and a wide linearity range from 5.0 × 10−7 to 1.0 × 10−4 M. It is biocompatible and highly stable on storage and under various pH conditions. Both the reaction and the SERS signal are highly specific over other species. The nanoprobe was successfully applied to SERS imaging of peroxynitrite that is produced in macrophages under oxidative stress. Conceivably, the method has a most viable tool for use in studies on peroxynitrite-related physiological and pathological processes. [Figure not available: see fulltext.].

KW - Biosensor

KW - Boronate ester

KW - Gold nanoparticles

KW - Living cell

KW - Oxidative stress

KW - Reactive oxygen species

KW - Specific reaction

KW - Surface-enhanced Raman scattering

UR - https://www.scopus.com/pages/publications/85058136923

U2 - 10.1007/s00604-018-3129-3

DO - 10.1007/s00604-018-3129-3

M3 - 文章

C2 - 30535866

AN - SCOPUS:85058136923

SN - 0026-3672

VL - 186

JO - Microchimica Acta

JF - Microchimica Acta

IS - 1

M1 - 11

ER -

A phenylboronate-based SERS nanoprobe for detection and imaging of intracellular peroxynitrite

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