A novel thin-layer amperometric detector based on chemically modified ring-disc electrode and its application for simultaneous measurements of nitric oxide and nitrite in rat brain combined with in vivo microdialysis

Lanqun Mao; Guoyue Shi; Yu Tian; Haiying Liu; Litong Jin; Katsunobu Yamamoto; Shuguang Tao; Jiye Jin

doi:10.1016/S0039-9140(98)00027-7

A novel thin-layer amperometric detector based on chemically modified ring-disc electrode and its application for simultaneous measurements of nitric oxide and nitrite in rat brain combined with in vivo microdialysis

Lanqun Mao, Guoyue Shi, Yu Tian, Haiying Liu, Litong Jin, Katsunobu Yamamoto, Shuguang Tao, Jiye Jin

School of Chemistry and Molecular Engineering

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

A novel thin-layer amperometric detector (TLAD) based on chemically modified ring-disc electrode and its application for simultaneous measurements of nitric oxide (NO) and nitrite (NO₂ ^-) in rat brain were demonstrated in this work. The ring-disc electrode was simultaneously sensitive to nitric oxide (NO) and nitrite (NO₂ ^-) by modifying its inner disc with electropolymerized film of cobalt(II) tetraaminophthalocyanine (polyCoTAPc)/Nafion and its outer ring with poly(vinylpyridine) (PVP), respectively. The ring-disc electrode was used to constitute a novel TLAD in radial flow cell for simultaneous measurements of NO and NO₂ ^- in rat brain combined with techniques of high performance liquid chromatography (HPLC) and in vivo microdialysis. It was found that the basal concentration of NO in the caudate nucleus of rat brain is lower than 1.0 x 10^-7 mol 1^-1, NO₂ ^- concentration is 5.0 x 10^-7 mol 1^-1 and NO exists in brain maybe mainly in the form of its decomposed product.

Original language	English
Pages (from-to)	1547-1556
Number of pages	10
Journal	Talanta
Volume	46
Issue number	6
DOIs	https://doi.org/10.1016/S0039-9140(98)00027-7
State	Published - Aug 1998

Keywords

Cobalt(II) tetraaminophthalocyanine
In vivo microdialysis
Nitric oxide
Nitrite
Poly(vinylpyridine)
Thin-layer amperometric detector

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Mao, L., Shi, G., Tian, Y., Liu, H., Jin, L., Yamamoto, K., Tao, S., & Jin, J. (1998). A novel thin-layer amperometric detector based on chemically modified ring-disc electrode and its application for simultaneous measurements of nitric oxide and nitrite in rat brain combined with in vivo microdialysis. Talanta, 46(6), 1547-1556. https://doi.org/10.1016/S0039-9140(98)00027-7

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title = "A novel thin-layer amperometric detector based on chemically modified ring-disc electrode and its application for simultaneous measurements of nitric oxide and nitrite in rat brain combined with in vivo microdialysis",

abstract = "A novel thin-layer amperometric detector (TLAD) based on chemically modified ring-disc electrode and its application for simultaneous measurements of nitric oxide (NO) and nitrite (NO2 -) in rat brain were demonstrated in this work. The ring-disc electrode was simultaneously sensitive to nitric oxide (NO) and nitrite (NO2 -) by modifying its inner disc with electropolymerized film of cobalt(II) tetraaminophthalocyanine (polyCoTAPc)/Nafion and its outer ring with poly(vinylpyridine) (PVP), respectively. The ring-disc electrode was used to constitute a novel TLAD in radial flow cell for simultaneous measurements of NO and NO2 - in rat brain combined with techniques of high performance liquid chromatography (HPLC) and in vivo microdialysis. It was found that the basal concentration of NO in the caudate nucleus of rat brain is lower than 1.0 x 10-7 mol 1-1, NO2 - concentration is 5.0 x 10-7 mol 1-1 and NO exists in brain maybe mainly in the form of its decomposed product.",

keywords = "Cobalt(II) tetraaminophthalocyanine, In vivo microdialysis, Nitric oxide, Nitrite, Poly(vinylpyridine), Thin-layer amperometric detector",

author = "Lanqun Mao and Guoyue Shi and Yu Tian and Haiying Liu and Litong Jin and Katsunobu Yamamoto and Shuguang Tao and Jiye Jin",

year = "1998",

month = aug,

doi = "10.1016/S0039-9140(98)00027-7",

language = "英语",

volume = "46",

pages = "1547--1556",

journal = "Talanta",

issn = "0039-9140",

publisher = "Elsevier B.V.",

number = "6",

}

Mao, L, Shi, G, Tian, Y, Liu, H, Jin, L, Yamamoto, K, Tao, S & Jin, J 1998, 'A novel thin-layer amperometric detector based on chemically modified ring-disc electrode and its application for simultaneous measurements of nitric oxide and nitrite in rat brain combined with in vivo microdialysis', Talanta, vol. 46, no. 6, pp. 1547-1556. https://doi.org/10.1016/S0039-9140(98)00027-7

A novel thin-layer amperometric detector based on chemically modified ring-disc electrode and its application for simultaneous measurements of nitric oxide and nitrite in rat brain combined with in vivo microdialysis. / Mao, Lanqun; Shi, Guoyue; Tian, Yu et al.
In: Talanta, Vol. 46, No. 6, 08.1998, p. 1547-1556.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A novel thin-layer amperometric detector based on chemically modified ring-disc electrode and its application for simultaneous measurements of nitric oxide and nitrite in rat brain combined with in vivo microdialysis

AU - Mao, Lanqun

AU - Shi, Guoyue

AU - Tian, Yu

AU - Liu, Haiying

AU - Jin, Litong

AU - Yamamoto, Katsunobu

AU - Tao, Shuguang

AU - Jin, Jiye

PY - 1998/8

Y1 - 1998/8

N2 - A novel thin-layer amperometric detector (TLAD) based on chemically modified ring-disc electrode and its application for simultaneous measurements of nitric oxide (NO) and nitrite (NO2 -) in rat brain were demonstrated in this work. The ring-disc electrode was simultaneously sensitive to nitric oxide (NO) and nitrite (NO2 -) by modifying its inner disc with electropolymerized film of cobalt(II) tetraaminophthalocyanine (polyCoTAPc)/Nafion and its outer ring with poly(vinylpyridine) (PVP), respectively. The ring-disc electrode was used to constitute a novel TLAD in radial flow cell for simultaneous measurements of NO and NO2 - in rat brain combined with techniques of high performance liquid chromatography (HPLC) and in vivo microdialysis. It was found that the basal concentration of NO in the caudate nucleus of rat brain is lower than 1.0 x 10-7 mol 1-1, NO2 - concentration is 5.0 x 10-7 mol 1-1 and NO exists in brain maybe mainly in the form of its decomposed product.

AB - A novel thin-layer amperometric detector (TLAD) based on chemically modified ring-disc electrode and its application for simultaneous measurements of nitric oxide (NO) and nitrite (NO2 -) in rat brain were demonstrated in this work. The ring-disc electrode was simultaneously sensitive to nitric oxide (NO) and nitrite (NO2 -) by modifying its inner disc with electropolymerized film of cobalt(II) tetraaminophthalocyanine (polyCoTAPc)/Nafion and its outer ring with poly(vinylpyridine) (PVP), respectively. The ring-disc electrode was used to constitute a novel TLAD in radial flow cell for simultaneous measurements of NO and NO2 - in rat brain combined with techniques of high performance liquid chromatography (HPLC) and in vivo microdialysis. It was found that the basal concentration of NO in the caudate nucleus of rat brain is lower than 1.0 x 10-7 mol 1-1, NO2 - concentration is 5.0 x 10-7 mol 1-1 and NO exists in brain maybe mainly in the form of its decomposed product.

KW - Cobalt(II) tetraaminophthalocyanine

KW - In vivo microdialysis

KW - Nitric oxide

KW - Nitrite

KW - Poly(vinylpyridine)

KW - Thin-layer amperometric detector

UR - https://www.scopus.com/pages/publications/0032145053

U2 - 10.1016/S0039-9140(98)00027-7

DO - 10.1016/S0039-9140(98)00027-7

M3 - 文章

AN - SCOPUS:0032145053

SN - 0039-9140

VL - 46

SP - 1547

EP - 1556

JO - Talanta

JF - Talanta

IS - 6

ER -

A novel thin-layer amperometric detector based on chemically modified ring-disc electrode and its application for simultaneous measurements of nitric oxide and nitrite in rat brain combined with in vivo microdialysis

Abstract

Keywords

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