A novel peptide from human apolipoprotein(a) Inhibits angiogenesls and tumor growth by targeting c-Src phosphorylation in VEGF-induced human umbilical endothelial cells

Many angiogenesis inhibitors are derived from large plasma proteins. Previous studies showed that the Kringle5-like domain (termed KV) in human apolipoprotein (a) is a potential antiangio-genic factor. However, its active region and the underling molecular mechanism remain elusive. Here, we identiied an 11-amino acid peptide (named KV11) as the key region for the antiangio-genie function of the KV domain of apolipoprotein (a). We demonstrate that KV11 inhibits angiogenesis in vitro by suppressing human umbilical vein endothelial cell migration and microtubule formation, KV 11 inhibits angiogenesis in chicken chorioallantoic membrane assays and mouse corneal micropocket angiogenesis assays in vivo. KV11 peptide shows no effect on tumor cell growth or proliferation, but significantly inhibits tumor growth in SCID mouse xenograft tumor model (p < 0.01) by preventing tumor angiogenesis. We elucidate that KV11 peptide suppresses angiogenesis and tumor progression by targeting the c-Src/ERK signaling pathways. Together, these studies provide the first evidence that KV 11 from apolipoprotein KV domain has anti-angiogenesis functions and may be an anti-tumor drug candidate.