TY - JOUR
T1 - A novel biosensor based on photoelectro-synergistic catalysis for flow-injection analysis system/amperometric detection of organophosphorous pesticides
AU - Wei, Yinyin
AU - Li, Ying
AU - Qu, Yunhe
AU - Xiao, Fei
AU - Shi, Guoyue
AU - Jin, Litong
PY - 2009/6/8
Y1 - 2009/6/8
N2 - In this study, a highly sensitive amperometric biosensor based on photoelectro-synergistic catalysis for detecting organophosphorus pesticides (OPs) in flow-injection analysis (FIA) system has been developed. The acetylcholinesterase enzyme (AChE) was immobilized by adsorption into the nanostructured PbO2/TiO2/Ti, which also acted as the working electrode. This strategy was found to catalyze the oxidative reaction of thiocholine effectively, make the AChE/PbO2/TiO2/Ti biosensor detect the substrate at 0.30 V (vs. SCE), hundreds milli-volts lower than others reported. PbO2/TiO2/Ti and TiO2/Ti electrodes were prepared and investigated with atomic force microscope (AFM). Factors influencing the performance were optimized. The resulting flow system offered a fast, sensitive, and stable response. A value of 1.34 mM for the apparent Michaelis-Menten constant (KMapp) was obtained. A wide linear inhibition response for trichlorfon was observed in the range of 0.01-20 μM with the detection limit of 0.1 nM. The results using this biosensor agreed very well with chromatographic method and we also examined the real samples successfully in this work.
AB - In this study, a highly sensitive amperometric biosensor based on photoelectro-synergistic catalysis for detecting organophosphorus pesticides (OPs) in flow-injection analysis (FIA) system has been developed. The acetylcholinesterase enzyme (AChE) was immobilized by adsorption into the nanostructured PbO2/TiO2/Ti, which also acted as the working electrode. This strategy was found to catalyze the oxidative reaction of thiocholine effectively, make the AChE/PbO2/TiO2/Ti biosensor detect the substrate at 0.30 V (vs. SCE), hundreds milli-volts lower than others reported. PbO2/TiO2/Ti and TiO2/Ti electrodes were prepared and investigated with atomic force microscope (AFM). Factors influencing the performance were optimized. The resulting flow system offered a fast, sensitive, and stable response. A value of 1.34 mM for the apparent Michaelis-Menten constant (KMapp) was obtained. A wide linear inhibition response for trichlorfon was observed in the range of 0.01-20 μM with the detection limit of 0.1 nM. The results using this biosensor agreed very well with chromatographic method and we also examined the real samples successfully in this work.
KW - Acetylcholinesterase
KW - Biosensor
KW - Organophosphorus pesticides
KW - Photoelectro-synergistic catalysis
UR - https://www.scopus.com/pages/publications/65549132838
U2 - 10.1016/j.aca.2009.03.045
DO - 10.1016/j.aca.2009.03.045
M3 - 文章
C2 - 19446058
AN - SCOPUS:65549132838
SN - 0003-2670
VL - 643
SP - 13
EP - 18
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 1-2
ER -