TY - JOUR
T1 - A novel alcohol dehydrogenase biosensor based on solid-state electrogenerated chemiluminescence by assembling dehydrogenase to Ru(bpy)32+-Au nanoparticles aggregates
AU - Zhang, Lihua
AU - Xu, Zhiai
AU - Sun, Xuping
AU - Dong, Shaojun
PY - 2007/1/15
Y1 - 2007/1/15
N2 - Based on electrogenerated chemiluminescence (ECL), a novel method for fabrication of alcohol dehydrogenase (ADH) biosensor by self-assembling ADH to Ru(bpy)32+-AuNPs aggregates (Ru-AuNPs) on indium tin oxide (ITO) electrode surface has been developed. Positively charged Ru(bpy)32+ could be immobilized stably on the electrode surface with negatively charged AuNPs in the form of aggregate via electrostatic interaction. On the other hand, AuNPs are favourable candidates for the immobilization of enzymes because amine groups and cysteine residues in the enzymes are known to bind strongly with AuNPs. Moreover, AuNPs can act as tiny conduction centers to facilitate the transfer of electrons. Such biosensor combined enzymatic selectivity with the sensitivity of ECL detection for quantification of enzyme substrate, and it displayed wide linear range, high sensitivity and good stability.
AB - Based on electrogenerated chemiluminescence (ECL), a novel method for fabrication of alcohol dehydrogenase (ADH) biosensor by self-assembling ADH to Ru(bpy)32+-AuNPs aggregates (Ru-AuNPs) on indium tin oxide (ITO) electrode surface has been developed. Positively charged Ru(bpy)32+ could be immobilized stably on the electrode surface with negatively charged AuNPs in the form of aggregate via electrostatic interaction. On the other hand, AuNPs are favourable candidates for the immobilization of enzymes because amine groups and cysteine residues in the enzymes are known to bind strongly with AuNPs. Moreover, AuNPs can act as tiny conduction centers to facilitate the transfer of electrons. Such biosensor combined enzymatic selectivity with the sensitivity of ECL detection for quantification of enzyme substrate, and it displayed wide linear range, high sensitivity and good stability.
KW - Alcohol dehydrogenase (ADH)
KW - Biosensor
KW - Electrogenerated chemiluminescence (ECL)
KW - Ru(bpy)-AuNPs aggregates (Ru-AuNPs)
UR - https://www.scopus.com/pages/publications/33845481630
U2 - 10.1016/j.bios.2006.03.026
DO - 10.1016/j.bios.2006.03.026
M3 - 文章
C2 - 16815003
AN - SCOPUS:33845481630
SN - 0956-5663
VL - 22
SP - 1097
EP - 1100
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 6
ER -