A MoS2-based system for efficient immobilization of hemoglobin and biosensing applications

Jie Chao; Min Zou; Chi Zhang; Haofan Sun; Dun Pan; Hao Pei; Shao Su; Lihui Yuwen; Chunhai Fan; Lianhui Wang

doi:10.1088/0957-4484/26/27/274005

A MoS₂-based system for efficient immobilization of hemoglobin and biosensing applications

Jie Chao
, Min Zou
, Chi Zhang
, Haofan Sun
, Dun Pan
, Hao Pei
, Shao Su
, Lihui Yuwen
, Chunhai Fan
, Lianhui Wang

Research output: Contribution to journal › Article › peer-review

68 Scopus citations

Abstract

A novel hydrogen peroxide (H₂O₂) and nitric oxide (NO) biosensor was fabricated by immobilizing hemoglobin (Hb) on a gold nanoparticle-decorated MoS₂ nanosheet (AuNPs@MoS₂) nanocomposite film modified glass carbon electrode. The AuNPs@MoS₂ nanocomposite not only made the immobilized Hb keep its native biological activity but also facilitated the electron transfer between electrode and the electroactive center of Hb due to its excellent conductivity and biocompatibility. The direct electrochemistry and bioelectrocatalytic activity of Hb were investigated by cyclic voltammetry (CV). The modified electrode showed good electrocatalytic ability toward the reduction of H₂O₂ and NO. Under optimal conditions, the current response was linear with the concentration of H₂O₂ and NO in the range from 10 to 300 μM and 10 to 1100 μM with a detection limit of 4 and 5 μM, respectively. This MoS₂-based biosensor was sensitive, reproducible and stable, indicating that AuNPs@MoS₂ nanocomposite maybe a promising platform to construct electrochemical sensors for chemical and biological molecules detection.

Original language	English
Article number	274005
Journal	Nanotechnology
Volume	26
Issue number	27
DOIs	https://doi.org/10.1088/0957-4484/26/27/274005
State	Published - 10 Jul 2015
Externally published	Yes

Keywords

AuNP@MS nanocomposite, electrochemical biosensor, hydrogen peroxide, nitric oxide

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10.1088/0957-4484/26/27/274005

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title = "A MoS2-based system for efficient immobilization of hemoglobin and biosensing applications",

abstract = "A novel hydrogen peroxide (H2O2) and nitric oxide (NO) biosensor was fabricated by immobilizing hemoglobin (Hb) on a gold nanoparticle-decorated MoS2 nanosheet (AuNPs@MoS2) nanocomposite film modified glass carbon electrode. The AuNPs@MoS2 nanocomposite not only made the immobilized Hb keep its native biological activity but also facilitated the electron transfer between electrode and the electroactive center of Hb due to its excellent conductivity and biocompatibility. The direct electrochemistry and bioelectrocatalytic activity of Hb were investigated by cyclic voltammetry (CV). The modified electrode showed good electrocatalytic ability toward the reduction of H2O2 and NO. Under optimal conditions, the current response was linear with the concentration of H2O2 and NO in the range from 10 to 300 μM and 10 to 1100 μM with a detection limit of 4 and 5 μM, respectively. This MoS2-based biosensor was sensitive, reproducible and stable, indicating that AuNPs@MoS2 nanocomposite maybe a promising platform to construct electrochemical sensors for chemical and biological molecules detection.",

keywords = "AuNP@MS nanocomposite, electrochemical biosensor, hydrogen peroxide, nitric oxide",

author = "Jie Chao and Min Zou and Chi Zhang and Haofan Sun and Dun Pan and Hao Pei and Shao Su and Lihui Yuwen and Chunhai Fan and Lianhui Wang",

note = "Publisher Copyright: {\textcopyright} 2015 IOP Publishing Ltd.",

year = "2015",

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doi = "10.1088/0957-4484/26/27/274005",

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TY - JOUR

T1 - A MoS2-based system for efficient immobilization of hemoglobin and biosensing applications

AU - Chao, Jie

AU - Zou, Min

AU - Zhang, Chi

AU - Sun, Haofan

AU - Pan, Dun

AU - Pei, Hao

AU - Su, Shao

AU - Yuwen, Lihui

AU - Fan, Chunhai

AU - Wang, Lianhui

PY - 2015/7/10

Y1 - 2015/7/10

N2 - A novel hydrogen peroxide (H2O2) and nitric oxide (NO) biosensor was fabricated by immobilizing hemoglobin (Hb) on a gold nanoparticle-decorated MoS2 nanosheet (AuNPs@MoS2) nanocomposite film modified glass carbon electrode. The AuNPs@MoS2 nanocomposite not only made the immobilized Hb keep its native biological activity but also facilitated the electron transfer between electrode and the electroactive center of Hb due to its excellent conductivity and biocompatibility. The direct electrochemistry and bioelectrocatalytic activity of Hb were investigated by cyclic voltammetry (CV). The modified electrode showed good electrocatalytic ability toward the reduction of H2O2 and NO. Under optimal conditions, the current response was linear with the concentration of H2O2 and NO in the range from 10 to 300 μM and 10 to 1100 μM with a detection limit of 4 and 5 μM, respectively. This MoS2-based biosensor was sensitive, reproducible and stable, indicating that AuNPs@MoS2 nanocomposite maybe a promising platform to construct electrochemical sensors for chemical and biological molecules detection.

AB - A novel hydrogen peroxide (H2O2) and nitric oxide (NO) biosensor was fabricated by immobilizing hemoglobin (Hb) on a gold nanoparticle-decorated MoS2 nanosheet (AuNPs@MoS2) nanocomposite film modified glass carbon electrode. The AuNPs@MoS2 nanocomposite not only made the immobilized Hb keep its native biological activity but also facilitated the electron transfer between electrode and the electroactive center of Hb due to its excellent conductivity and biocompatibility. The direct electrochemistry and bioelectrocatalytic activity of Hb were investigated by cyclic voltammetry (CV). The modified electrode showed good electrocatalytic ability toward the reduction of H2O2 and NO. Under optimal conditions, the current response was linear with the concentration of H2O2 and NO in the range from 10 to 300 μM and 10 to 1100 μM with a detection limit of 4 and 5 μM, respectively. This MoS2-based biosensor was sensitive, reproducible and stable, indicating that AuNPs@MoS2 nanocomposite maybe a promising platform to construct electrochemical sensors for chemical and biological molecules detection.

KW - AuNP@MS nanocomposite, electrochemical biosensor, hydrogen peroxide, nitric oxide

UR - https://www.scopus.com/pages/publications/84935002289

U2 - 10.1088/0957-4484/26/27/274005

DO - 10.1088/0957-4484/26/27/274005

M3 - 文章

C2 - 26087018

AN - SCOPUS:84935002289

SN - 0957-4484

VL - 26

JO - Nanotechnology

JF - Nanotechnology

IS - 27

M1 - 274005

ER -

A MoS₂-based system for efficient immobilization of hemoglobin and biosensing applications

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