A highly soluble poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid)/Au nanocomposite for horseradish peroxidase immobilization and biosensing

Jingjing Xu; Ru Peng; Qin Ran; Yuezhong Xian; Yuan Tian; Litong Jin

doi:10.1016/j.talanta.2010.07.033

A highly soluble poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid)/Au nanocomposite for horseradish peroxidase immobilization and biosensing

Jingjing Xu
, Ru Peng
, Qin Ran
, Yuezhong Xian^*
, Yuan Tian
, Litong Jin

^*Corresponding author for this work

School of Chemistry and Molecular Engineering

East China Normal University

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

A highly soluble poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid)/Au (PEDOT-PSS/Au) nanocomposite was prepared via one-step chemical synthesis and the matrix was characterized by UV-vis spectroscopy (UV-vis), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and transmission electron microscope (TEM). Due to the excellent aqueous compatibility and biocompatibility, the PEDOT/PSS-Au nanocomposite can be used as biomaterial for enzymes immobilization. In this system, redox enzyme, horseradish peroxidase (HRP) was integrated with PEDOT/PSS-Au nanocomposite and the direct electron transfer of HRP was observed. Moreover, we find that the HRP/PEDOT-PSS/Au modified electrode shows excellent electrocatalytic ability for H₂O₂ and the formal Michaelis-Menten constant (Kmapp) was 0.78 mmol/L. The response currents have good linear relation with the concentrations of H₂O₂ with a linear range from 2.0 × 10^-7 to 3.8 × 10^-4 mol/L.

Original language	English
Pages (from-to)	1511-1515
Number of pages	5
Journal	Talanta
Volume	82
Issue number	4
DOIs	https://doi.org/10.1016/j.talanta.2010.07.033
State	Published - 15 Sep 2010

Keywords

Au nanopartiles
Biosensor
Direct electrochemistry
Horseradish peroxidase
Hydrogen peroxide
PEDOT-PSS conductive polymer

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10.1016/j.talanta.2010.07.033

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title = "A highly soluble poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid)/Au nanocomposite for horseradish peroxidase immobilization and biosensing",

abstract = "A highly soluble poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid)/Au (PEDOT-PSS/Au) nanocomposite was prepared via one-step chemical synthesis and the matrix was characterized by UV-vis spectroscopy (UV-vis), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and transmission electron microscope (TEM). Due to the excellent aqueous compatibility and biocompatibility, the PEDOT/PSS-Au nanocomposite can be used as biomaterial for enzymes immobilization. In this system, redox enzyme, horseradish peroxidase (HRP) was integrated with PEDOT/PSS-Au nanocomposite and the direct electron transfer of HRP was observed. Moreover, we find that the HRP/PEDOT-PSS/Au modified electrode shows excellent electrocatalytic ability for H2O2 and the formal Michaelis-Menten constant (Kmapp) was 0.78 mmol/L. The response currents have good linear relation with the concentrations of H2O2 with a linear range from 2.0 × 10-7 to 3.8 × 10-4 mol/L.",

keywords = "Au nanopartiles, Biosensor, Direct electrochemistry, Horseradish peroxidase, Hydrogen peroxide, PEDOT-PSS conductive polymer",

author = "Jingjing Xu and Ru Peng and Qin Ran and Yuezhong Xian and Yuan Tian and Litong Jin",

year = "2010",

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doi = "10.1016/j.talanta.2010.07.033",

language = "英语",

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T1 - A highly soluble poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid)/Au nanocomposite for horseradish peroxidase immobilization and biosensing

AU - Xu, Jingjing

AU - Peng, Ru

AU - Ran, Qin

AU - Xian, Yuezhong

AU - Tian, Yuan

AU - Jin, Litong

PY - 2010/9/15

Y1 - 2010/9/15

N2 - A highly soluble poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid)/Au (PEDOT-PSS/Au) nanocomposite was prepared via one-step chemical synthesis and the matrix was characterized by UV-vis spectroscopy (UV-vis), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and transmission electron microscope (TEM). Due to the excellent aqueous compatibility and biocompatibility, the PEDOT/PSS-Au nanocomposite can be used as biomaterial for enzymes immobilization. In this system, redox enzyme, horseradish peroxidase (HRP) was integrated with PEDOT/PSS-Au nanocomposite and the direct electron transfer of HRP was observed. Moreover, we find that the HRP/PEDOT-PSS/Au modified electrode shows excellent electrocatalytic ability for H2O2 and the formal Michaelis-Menten constant (Kmapp) was 0.78 mmol/L. The response currents have good linear relation with the concentrations of H2O2 with a linear range from 2.0 × 10-7 to 3.8 × 10-4 mol/L.

AB - A highly soluble poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid)/Au (PEDOT-PSS/Au) nanocomposite was prepared via one-step chemical synthesis and the matrix was characterized by UV-vis spectroscopy (UV-vis), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and transmission electron microscope (TEM). Due to the excellent aqueous compatibility and biocompatibility, the PEDOT/PSS-Au nanocomposite can be used as biomaterial for enzymes immobilization. In this system, redox enzyme, horseradish peroxidase (HRP) was integrated with PEDOT/PSS-Au nanocomposite and the direct electron transfer of HRP was observed. Moreover, we find that the HRP/PEDOT-PSS/Au modified electrode shows excellent electrocatalytic ability for H2O2 and the formal Michaelis-Menten constant (Kmapp) was 0.78 mmol/L. The response currents have good linear relation with the concentrations of H2O2 with a linear range from 2.0 × 10-7 to 3.8 × 10-4 mol/L.

KW - Au nanopartiles

KW - Biosensor

KW - Direct electrochemistry

KW - Horseradish peroxidase

KW - Hydrogen peroxide

KW - PEDOT-PSS conductive polymer

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U2 - 10.1016/j.talanta.2010.07.033

DO - 10.1016/j.talanta.2010.07.033

M3 - 文章

AN - SCOPUS:77956186986

SN - 0039-9140

VL - 82

SP - 1511

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JO - Talanta

JF - Talanta

IS - 4

ER -

A highly soluble poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid)/Au nanocomposite for horseradish peroxidase immobilization and biosensing

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