A highly sensitive and selective nanosensor for near-infrared potassium imaging

Potassium ion (K⁺) concentration fluctuates in various biological processes. A number of K⁺ probes have been developed to monitor such fluctuations through optical imaging. However, the currently available K⁺ probes are far from being sensitive enough in detecting physiological fluctuations in living animals. Furthermore, the monitoring of deep tissues is not applicable because of short-wavelength excitation prevailingly used so far. Here, we report a highly sensitive and selective nanosensor for near-infrared (NIR) K⁺ imaging in living cells and animals. The nanosensor is constructed by encapsulating upconversion nanoparticles (UCNPs) and a commercial K⁺ indicator in the hollow cavity of mesoporous silica nanoparticles, followed by coating a K⁺-selective filter membrane. The membrane adsorbs K⁺ from the medium and filters out interfering cations. The UCNPs convert NIR to ultraviolet light, which excites the K⁺ indicator, thus allowing the detection of the fluctuations of K⁺ concentration in cultured cells and intact mouse brains.