TY - JOUR
T1 - A dual signal amplification strategy based on scanning electrochemical microscopy for DNA biosensing using a PEDOT-modified ultramicroelectrode and long self-assembled DNA concatemers
AU - Ning, Xin
AU - Xiong, Qiang
AU - Wu, Tao
AU - Zhang, Fan
AU - He, Pingang
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/7/1
Y1 - 2019/7/1
N2 - A PEDOT-modified Pt microelectrode was used in SECM for DNA biosensing and combined with long self-assembled DNA concatemers to realize dual signal amplification. The approach curve could also be plotted for tip location using this modified microelectrode with no impact on the catalytic effect so that both signal amplification and tip locating were achieved in one single tip. The detection limit of this method reached 0.076 fM, providing an ultra-sensitive DNA biosensing platform. Furthermore, this strategy could be applied in scanning a DNA chip, showing great potential in high-throughput bioanalysis with high sensitivity.
AB - A PEDOT-modified Pt microelectrode was used in SECM for DNA biosensing and combined with long self-assembled DNA concatemers to realize dual signal amplification. The approach curve could also be plotted for tip location using this modified microelectrode with no impact on the catalytic effect so that both signal amplification and tip locating were achieved in one single tip. The detection limit of this method reached 0.076 fM, providing an ultra-sensitive DNA biosensing platform. Furthermore, this strategy could be applied in scanning a DNA chip, showing great potential in high-throughput bioanalysis with high sensitivity.
KW - Dual signal amplification
KW - PEDOT-modified ultramicroelectrode
KW - Scanning electrochemical microscopy
UR - https://www.scopus.com/pages/publications/85063864918
U2 - 10.1016/j.snb.2019.03.128
DO - 10.1016/j.snb.2019.03.128
M3 - 文章
AN - SCOPUS:85063864918
SN - 0925-4005
VL - 290
SP - 371
EP - 377
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
ER -