A dual-reactivity-based surface-enhanced Raman spectroscopy nanosensor for the simultaneous imaging of hypochlorite and nitric oxide in living cells

Hua Ying Chen; Yue He; Chao Chen; Jing Wang; Kai Ge; Bin Bin Chen; Da Wei Li

doi:10.1016/j.saa.2025.125899

A dual-reactivity-based surface-enhanced Raman spectroscopy nanosensor for the simultaneous imaging of hypochlorite and nitric oxide in living cells

Hua Ying Chen^*
, Yue He
, Chao Chen
, Jing Wang
, Kai Ge
, Bin Bin Chen
, Da Wei Li

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A surface-enhanced Raman spectroscopy (SERS) nanosensor with dual-reactivity is developed for the simultaneous imaging of hypochlorite (ClO⁻) and nitric oxide (NO) in living cells. Utilizing the specific reactions between functional molecules and ClO⁻ and NO, respectively, the 2-mercapto-4-methoxy-phenol (2-MP) and o-phenylenediamine (OPD) molecules are synchronously assembled on the surface of gold nanoparticles to fabricate the dual-function nanosensors. The advantages of SERS technology, narrow peaks for spectral multiplexing and fingerprint information, further facilitate the simultaneous detection of ClO⁻ and NO. The prepared nanosensors achieve a highly sensitive and selective measurement of ClO⁻ and NO with a limit of detection of 0.054 μM and 0.46 μM, respectively. Furthermore, the SERS nanosensors enable the simultaneous visualization of ClO⁻ and NO in the single living cell, which opens up the prospects to investigate the ClO⁻- and NO-involved physiological and pathological events.

Original language	English
Article number	125899
Journal	Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume	334
DOIs	https://doi.org/10.1016/j.saa.2025.125899
State	Published - 5 Jun 2025
Externally published	Yes

Keywords

Nanosensors
ROS/RNS
SERS
Simultaneous detection
Single-cell imaging analysis

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10.1016/j.saa.2025.125899

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@article{b6da2ac443944776948567978f9ebc8c,

title = "A dual-reactivity-based surface-enhanced Raman spectroscopy nanosensor for the simultaneous imaging of hypochlorite and nitric oxide in living cells",

abstract = "A surface-enhanced Raman spectroscopy (SERS) nanosensor with dual-reactivity is developed for the simultaneous imaging of hypochlorite (ClO−) and nitric oxide (NO) in living cells. Utilizing the specific reactions between functional molecules and ClO− and NO, respectively, the 2-mercapto-4-methoxy-phenol (2-MP) and o-phenylenediamine (OPD) molecules are synchronously assembled on the surface of gold nanoparticles to fabricate the dual-function nanosensors. The advantages of SERS technology, narrow peaks for spectral multiplexing and fingerprint information, further facilitate the simultaneous detection of ClO− and NO. The prepared nanosensors achieve a highly sensitive and selective measurement of ClO− and NO with a limit of detection of 0.054 μM and 0.46 μM, respectively. Furthermore, the SERS nanosensors enable the simultaneous visualization of ClO− and NO in the single living cell, which opens up the prospects to investigate the ClO−- and NO-involved physiological and pathological events.",

keywords = "Nanosensors, ROS/RNS, SERS, Simultaneous detection, Single-cell imaging analysis",

author = "Chen, \{Hua Ying\} and Yue He and Chao Chen and Jing Wang and Kai Ge and Chen, \{Bin Bin\} and Li, \{Da Wei\}",

note = "Publisher Copyright: {\textcopyright} 2025 Elsevier B.V.",

year = "2025",

month = jun,

day = "5",

doi = "10.1016/j.saa.2025.125899",

language = "英语",

volume = "334",

journal = "Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy",

issn = "1386-1425",

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TY - JOUR

T1 - A dual-reactivity-based surface-enhanced Raman spectroscopy nanosensor for the simultaneous imaging of hypochlorite and nitric oxide in living cells

AU - Chen, Hua Ying

AU - He, Yue

AU - Chen, Chao

AU - Wang, Jing

AU - Ge, Kai

AU - Chen, Bin Bin

AU - Li, Da Wei

PY - 2025/6/5

Y1 - 2025/6/5

N2 - A surface-enhanced Raman spectroscopy (SERS) nanosensor with dual-reactivity is developed for the simultaneous imaging of hypochlorite (ClO−) and nitric oxide (NO) in living cells. Utilizing the specific reactions between functional molecules and ClO− and NO, respectively, the 2-mercapto-4-methoxy-phenol (2-MP) and o-phenylenediamine (OPD) molecules are synchronously assembled on the surface of gold nanoparticles to fabricate the dual-function nanosensors. The advantages of SERS technology, narrow peaks for spectral multiplexing and fingerprint information, further facilitate the simultaneous detection of ClO− and NO. The prepared nanosensors achieve a highly sensitive and selective measurement of ClO− and NO with a limit of detection of 0.054 μM and 0.46 μM, respectively. Furthermore, the SERS nanosensors enable the simultaneous visualization of ClO− and NO in the single living cell, which opens up the prospects to investigate the ClO−- and NO-involved physiological and pathological events.

AB - A surface-enhanced Raman spectroscopy (SERS) nanosensor with dual-reactivity is developed for the simultaneous imaging of hypochlorite (ClO−) and nitric oxide (NO) in living cells. Utilizing the specific reactions between functional molecules and ClO− and NO, respectively, the 2-mercapto-4-methoxy-phenol (2-MP) and o-phenylenediamine (OPD) molecules are synchronously assembled on the surface of gold nanoparticles to fabricate the dual-function nanosensors. The advantages of SERS technology, narrow peaks for spectral multiplexing and fingerprint information, further facilitate the simultaneous detection of ClO− and NO. The prepared nanosensors achieve a highly sensitive and selective measurement of ClO− and NO with a limit of detection of 0.054 μM and 0.46 μM, respectively. Furthermore, the SERS nanosensors enable the simultaneous visualization of ClO− and NO in the single living cell, which opens up the prospects to investigate the ClO−- and NO-involved physiological and pathological events.

KW - Nanosensors

KW - ROS/RNS

KW - SERS

KW - Simultaneous detection

KW - Single-cell imaging analysis

UR - https://www.scopus.com/pages/publications/85218110937

U2 - 10.1016/j.saa.2025.125899

DO - 10.1016/j.saa.2025.125899

M3 - 文章

C2 - 39983277

AN - SCOPUS:85218110937

SN - 1386-1425

VL - 334

JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

M1 - 125899

ER -

A dual-reactivity-based surface-enhanced Raman spectroscopy nanosensor for the simultaneous imaging of hypochlorite and nitric oxide in living cells

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Keywords

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