A DPA-based highly selective and sensitive fluorescent probe for mercuric ions and its imaging in living cells

Junhua Jia; Luling Wu; Yu Ding; Chusen Huang; Weiping Zhu; Yufang Xu; Xuhong Qian

doi:10.1039/c6dt01258b

A DPA-based highly selective and sensitive fluorescent probe for mercuric ions and its imaging in living cells

Junhua Jia
, Luling Wu
, Yu Ding
, Chusen Huang^*
, Weiping Zhu
, Yufang Xu
, Xuhong Qian

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Based on the 2,2-dipicolylamine (DPA) receptor and naphthalimide fluorophore, three fluorescent probes, RDPA, MDPA and VDPA have been developed for the recognition of mercuric ions. Among them, VDPA, bearing a diglycol group in the imine chain, exhibits good water solubility, and high selectivity towards mercuric ions in PBS water solution. With DPA as the receptor, VDPA showed very high affinity and sensitivity towards Hg²⁺, and the binding constant and detection limit were calculated to be 2.8 × 10¹⁰ M^-1 and 5.49 nM, respectively. The MTT assay and living cell imaging experiments suggested that the probe VDPA has potential application for detecting Hg²⁺ in living cells.

Original language	English
Pages (from-to)	9402-9406
Number of pages	5
Journal	Dalton Transactions
Volume	45
Issue number	23
DOIs	https://doi.org/10.1039/c6dt01258b
State	Published - 2016
Externally published	Yes

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title = "A DPA-based highly selective and sensitive fluorescent probe for mercuric ions and its imaging in living cells",

abstract = "Based on the 2,2-dipicolylamine (DPA) receptor and naphthalimide fluorophore, three fluorescent probes, RDPA, MDPA and VDPA have been developed for the recognition of mercuric ions. Among them, VDPA, bearing a diglycol group in the imine chain, exhibits good water solubility, and high selectivity towards mercuric ions in PBS water solution. With DPA as the receptor, VDPA showed very high affinity and sensitivity towards Hg2+, and the binding constant and detection limit were calculated to be 2.8 × 1010 M-1 and 5.49 nM, respectively. The MTT assay and living cell imaging experiments suggested that the probe VDPA has potential application for detecting Hg2+ in living cells.",

author = "Junhua Jia and Luling Wu and Yu Ding and Chusen Huang and Weiping Zhu and Yufang Xu and Xuhong Qian",

note = "Publisher Copyright: {\textcopyright} 2016 The Royal Society of Chemistry.",

year = "2016",

doi = "10.1039/c6dt01258b",

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TY - JOUR

T1 - A DPA-based highly selective and sensitive fluorescent probe for mercuric ions and its imaging in living cells

AU - Jia, Junhua

AU - Wu, Luling

AU - Ding, Yu

AU - Huang, Chusen

AU - Zhu, Weiping

AU - Xu, Yufang

AU - Qian, Xuhong

PY - 2016

Y1 - 2016

N2 - Based on the 2,2-dipicolylamine (DPA) receptor and naphthalimide fluorophore, three fluorescent probes, RDPA, MDPA and VDPA have been developed for the recognition of mercuric ions. Among them, VDPA, bearing a diglycol group in the imine chain, exhibits good water solubility, and high selectivity towards mercuric ions in PBS water solution. With DPA as the receptor, VDPA showed very high affinity and sensitivity towards Hg2+, and the binding constant and detection limit were calculated to be 2.8 × 1010 M-1 and 5.49 nM, respectively. The MTT assay and living cell imaging experiments suggested that the probe VDPA has potential application for detecting Hg2+ in living cells.

AB - Based on the 2,2-dipicolylamine (DPA) receptor and naphthalimide fluorophore, three fluorescent probes, RDPA, MDPA and VDPA have been developed for the recognition of mercuric ions. Among them, VDPA, bearing a diglycol group in the imine chain, exhibits good water solubility, and high selectivity towards mercuric ions in PBS water solution. With DPA as the receptor, VDPA showed very high affinity and sensitivity towards Hg2+, and the binding constant and detection limit were calculated to be 2.8 × 1010 M-1 and 5.49 nM, respectively. The MTT assay and living cell imaging experiments suggested that the probe VDPA has potential application for detecting Hg2+ in living cells.

UR - https://www.scopus.com/pages/publications/84973901426

U2 - 10.1039/c6dt01258b

DO - 10.1039/c6dt01258b

M3 - 文章

C2 - 27221695

AN - SCOPUS:84973901426

SN - 1477-9226

VL - 45

SP - 9402

EP - 9406

JO - Dalton Transactions

JF - Dalton Transactions

IS - 23

ER -

A DPA-based highly selective and sensitive fluorescent probe for mercuric ions and its imaging in living cells

Abstract

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