TY - JOUR
T1 - A craphene nanoprobe for rapid, sensitive, and multicolor fluorescent DNA analysis
AU - He, Shijiang
AU - Song, Bo
AU - Li, Di
AU - Zhu, Changfeng
AU - Qi, Wenpeng
AU - Wen, Yanqin
AU - Wang, Lihua
AU - Song, Shiping
AU - Fang, Haiping
AU - Fan, Chunhai
PY - 2010/2/8
Y1 - 2010/2/8
N2 - Coupling nanomaterials with biomolecular recognition events represents a new direction in nanotechnology toward the development of novel molecular diagnostic tools. Here a graphene oxide (CO)-based multicolor fluorescent DNA nanoprobe that allows rapid, sensitive, and selective detection of DNA targets in homogeneous solution by exploiting interactions between GO and DNA molecules is reported. Because of the extraordinarily high quenching efficiency of GO, the fluorescent ssDNA probe exhibits minimal background fluorescence, while strong emission is observed when it forms a double helix with the specific targets, leading to a high signal-to-background ratio. Importantly, the large planar surface of GO allows simultaneous quenching of multiple DNA probes labeled with different dyes, leading to a multicolor sensor for the detection of multiple DNA targets in the same solution. It is also demonstrated that this GO-based sensing platform is suitable for the detection of a range of analytes when complemented with the use of functional DNA structures.
AB - Coupling nanomaterials with biomolecular recognition events represents a new direction in nanotechnology toward the development of novel molecular diagnostic tools. Here a graphene oxide (CO)-based multicolor fluorescent DNA nanoprobe that allows rapid, sensitive, and selective detection of DNA targets in homogeneous solution by exploiting interactions between GO and DNA molecules is reported. Because of the extraordinarily high quenching efficiency of GO, the fluorescent ssDNA probe exhibits minimal background fluorescence, while strong emission is observed when it forms a double helix with the specific targets, leading to a high signal-to-background ratio. Importantly, the large planar surface of GO allows simultaneous quenching of multiple DNA probes labeled with different dyes, leading to a multicolor sensor for the detection of multiple DNA targets in the same solution. It is also demonstrated that this GO-based sensing platform is suitable for the detection of a range of analytes when complemented with the use of functional DNA structures.
UR - https://www.scopus.com/pages/publications/76149090494
U2 - 10.1002/adfm.200901639
DO - 10.1002/adfm.200901639
M3 - 文章
AN - SCOPUS:76149090494
SN - 1616-301X
VL - 20
SP - 453
EP - 459
JO - Advanced Functional Materials
JF - Advanced Functional Materials
IS - 3
ER -