TY - JOUR
T1 - 3D organoids derived from the small intestine
T2 - An emerging tool for drug transport research
AU - Zhang, Yuanjin
AU - Huang, Shengbo
AU - Zhong, Weiguo
AU - Chen, Wenxia
AU - Yao, Bingyi
AU - Wang, Xin
N1 - Publisher Copyright:
© 2021 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences
PY - 2021/7
Y1 - 2021/7
N2 - Small intestine in vitro models play a crucial role in drug transport research. Although conventional 2D cell culture models, such as Caco-2 monolayer, possess many advantages, they should be interpreted with caution because they have relatively poor physiologically reproducible phenotypes and functions. With the development of 3D culture technology, pluripotent stem cells (PSCs) and adult somatic stem cells (ASCs) show remarkable self-organization characteristics, which leads to the development of intestinal organoids. Based on previous studies, this paper reviews the application of intestinal 3D organoids in drug transport mediated by P-glycoprotein (P-gp), breast cancer resistance protein (BCRP) and multidrug resistance protein 2 (MRP2). The advantages and limitations of this model are also discussed. Although there are still many challenges, intestinal 3D organoid model has the potential to be an excellent tool for drug transport research.
AB - Small intestine in vitro models play a crucial role in drug transport research. Although conventional 2D cell culture models, such as Caco-2 monolayer, possess many advantages, they should be interpreted with caution because they have relatively poor physiologically reproducible phenotypes and functions. With the development of 3D culture technology, pluripotent stem cells (PSCs) and adult somatic stem cells (ASCs) show remarkable self-organization characteristics, which leads to the development of intestinal organoids. Based on previous studies, this paper reviews the application of intestinal 3D organoids in drug transport mediated by P-glycoprotein (P-gp), breast cancer resistance protein (BCRP) and multidrug resistance protein 2 (MRP2). The advantages and limitations of this model are also discussed. Although there are still many challenges, intestinal 3D organoid model has the potential to be an excellent tool for drug transport research.
KW - 3D organoid
KW - Caco-2 cell monolayer
KW - Drug transporter
KW - P-glycoprotein
KW - Small intestine
UR - https://www.scopus.com/pages/publications/85099129638
U2 - 10.1016/j.apsb.2020.12.002
DO - 10.1016/j.apsb.2020.12.002
M3 - 文献综述
AN - SCOPUS:85099129638
SN - 2211-3835
VL - 11
SP - 1697
EP - 1707
JO - Acta Pharmaceutica Sinica B
JF - Acta Pharmaceutica Sinica B
IS - 7
ER -
